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- W1976875510 abstract "Abstract Forty‐seven saturated synthetic diacyl PA, PC, PE, PG, and PS and five unsaturated diacyl phospholipids (PL) underwent normal‐ and reversed‐phase (RP) HPLC with isocratic isopropanol/hexane/water (5∶4∶1) and methanol/chloroform/acetonitrile/water (79.5∶9∶8∶3.5) mobile phases, respectively. For normal‐phase HPLC, capacity factors ( k′ i ) decrease with chain length ( n ) of the two identical PL FA residues, whereas the opposite occurs with RP (C 18 )‐HPLC. Plots of In k′ i vs. n for individual PL classes are in general curved, violating the linear free‐energy relationship. For PL of the same n but with different head groups, k′ i with normal‐phase HPLC varies as PE<PG<PA<PS<PC, except when n ≥16, when the order is PE<PS≈PA≈PG<PC. For RP‐HPLC, the order of k′ i values is PG<_A≈PS≤PC≈PE until n ≥16, when it is PA≈PG<PS≪PC≈PE. With normal‐phase HPLC, k′ i values of PL with unsaturated FA of n =18 are ordered as PE<PA<PC. Increasing degrees of unsaturation lead to increasing k′ i ." @default.
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- W1976875510 date "2002-12-01" @default.
- W1976875510 modified "2023-09-29" @default.
- W1976875510 title "Effect of FA chain length on normal- and reversed-phase HPLC of phospholipids" @default.
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- W1976875510 doi "https://doi.org/10.1007/s11746-002-0625-0" @default.
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