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- W1976946119 startingPage "257" @default.
- W1976946119 abstract "A simple separation method involving isocratic elution and a selective postcolumn derivatization method involving chemical oxidation followed by a fluorescence reaction are described regarding a simultaneous high performance liquid chromatographic determination of catecholamines (three species) and their precursor (one species) and metabolites (nine species, all in the free form). These compounds, isoproterenol and 3,4-dihydroxyphenylpropanoic acid, are separated by ion-pair reversed-phase chromatography using a mixture of citrate buffer (pH 2,5) containing sodium octanesulfonate and methanol; they are then oxidized with periodate to the corresponding o-quinones, which are converted into fluorescent derivatives by reactions with meso-1,2 Hliphenylethylenediamine. The detection limits (S/N=3, on column) vary from 15 to 570 fmol, depending on the compounds." @default.
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- W1976946119 date "1991-04-01" @default.
- W1976946119 modified "2023-09-27" @default.
- W1976946119 title "Simultaneous Determination of Catecholamine-Related Compounds by High Performance Liquid Chromatography with Postcolumn Chemical Oxidation Followed by a Fluorescence Reaction" @default.
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- W1976946119 doi "https://doi.org/10.2116/analsci.7.257" @default.
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