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- W1976989760 abstract "There are many research shows that the umbilical cord was a “store house” of stem cells. Human umbilical cord mesenchymal stem cell(HUMSC) was considered to be an idea seed cell for cell therapy and cell models construction used in drug development. But the regulation of HUMSC proliferation and differentiation in vitro is facing challenges. This research aimed to construct a biomimic microenvironment using bioactive materials including the fusion protein of E-cadherin-Fc and promote HUMSC proliferation. The adhersion and proliferation of HUMSC measured by MTT assay, were obviously enhanced on E-cadherin-Fc coated surfaces, comparing with these on the gelatin coated surfaces and conventional conditions. Meanwhile, the expression of CD105 assayed by flow cytometry, showing that the pluripotency of HUMSC cultured on the E-cadherin-Fc coated surfaces were higher than that in the tissue treated plate. From the above, the establishment of E-cadherin-Fc microenvionment is beneficial for HUMSC proliferation in vitro." @default.
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- W1976989760 date "2012-07-01" @default.
- W1976989760 modified "2023-09-24" @default.
- W1976989760 title "Promoting the Expansion of Human Umbilical Cord Mesenchymal Stem Cell <i>In Vitro</i> by E-Cadherin-Fc as a New Smart Biological Materials" @default.
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- W1976989760 doi "https://doi.org/10.4028/www.scientific.net/amr.554-556.1785" @default.
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