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• W1976994980 abstract "<i>Objective:</i> To develop a method for immunohistochemical staining of three different T lymphocyte antigens (CD4 or CD8, CD57 and TCRβ) on the same tissue section and to determine whether tissues have been infiltrated with T lymphocytes expressing these markers. <i>Study Design:</i> Commercially available antibodies were tested for immunohistochemical usefulness in a dye-based conventional single-immunostaining method after antigen retrieval on paraffin-embedded human lymph nodes. We searched for the combination of antibodies that could detect T lymphocyte antigens on the same section without any cross-reactivity and that have fluorescent signals robust enough to overcome paraffin autofluorescence. <i>Results:</i> Application of the antigen retrieval technique and the Sudan black B quenching technique enabled staining of paraffin-embedded tissue sections with fluorescent-labeled secondary antibodies. The combination of primary and secondary antibodies that could simultaneously detect the T lymphocyte antigens CD4 or CD8, CD57 and TCRβ in histochemical analysis of a paraffin-embedded human lymph node section was established, and was successfully applied to a human tissue section infiltrated with T lymphocytes that express these markers. <i>Conclusion:</i> The antibodies listed here would be helpful for histopathologists who wish to investigate T lymphocytes in the paraffin-embedded sections that have accumulated in pathology labs throughout the world." @default.
• W1976994980 created "2016-06-24" @default.
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• W1976994980 date "2011-01-01" @default.
• W1976994980 modified "2023-09-23" @default.
• W1976994980 title "Simultaneous Detection of T Lymphocyte-Related Antigens (CD4/CD8, CD57, TCRβ) with Nuclei by Fluorescence-Based Immunohistochemistry in Paraffin-Embedded Human Lymph Node, Liver Cancer and Stomach Cancer" @default.
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• W1976994980 doi "https://doi.org/10.1159/000329487" @default.
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