FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1977030002> ?p ?o ?g. }

• W1977030002 endingPage "455" @default.
• W1977030002 startingPage "449" @default.
• W1977030002 abstract "Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis throughout postnatal life in male and have the ability to transmit genetic information to the subsequent generation. In this study, we have optimized the transduction efficiency of SSCs using a lentiviral vector by considering different multiplicity of infection (MOI), duration of infection, presence or absence of feeder layer and polycationic agents. We tested MOI of 5, 10 or 20 and infection duration of 6, 9 or 12 h respectively. After infection, cells were cultured for 1 week and as a result, the number of transduced SSCs increased significantly for MOI of 5 and 10 with 6 h of infection. When the same condition (MOI of 5 with 6 hours) was applied in presence or absence of STO feeder layer and infected SSCs were cultured for 3 weeks on the STO feeder layer, a significant increase in the number of transduced cells was observed for without the feeder layer during infection. We subsequently studied the effects of polycationic agents, polybrene and dioctadecylamidoglycyl spermine (DOGS), on the transduction efficiency. Compared with the polybrene treatment, the recovery rate of the transduced SSCs was significantly higher for the DOGS treatment. Therefore, our optimization study could contribute to the enhancement of germ-line modification of SSCs using lentiviral vectors and in generation of transgenic animals." @default.
• W1977030002 created "2016-06-24" @default.
• W1977030002 creator A5001931943 @default.
• W1977030002 creator A5004021907 @default.
• W1977030002 creator A5011702150 @default.
• W1977030002 creator A5014649009 @default.
• W1977030002 creator A5027062877 @default.
• W1977030002 creator A5034805413 @default.
• W1977030002 creator A5044988065 @default.
• W1977030002 creator A5062342062 @default.
• W1977030002 creator A5077798472 @default.
• W1977030002 date "2012-04-17" @default.
• W1977030002 modified "2023-10-16" @default.
• W1977030002 title "Efficient enhancement of lentiviral transduction efficiency in murine spermatogonial stem cells" @default.
• W1977030002 cites W1971290197 @default.
• W1977030002 cites W1978397931 @default.
• W1977030002 cites W1990205198 @default.
• W1977030002 cites W1993491850 @default.
• W1977030002 cites W2016789158 @default.
• W1977030002 cites W2021971921 @default.
• W1977030002 cites W2037755687 @default.
• W1977030002 cites W2041208222 @default.
• W1977030002 cites W2045847970 @default.
• W1977030002 cites W2045887697 @default.
• W1977030002 cites W2060217553 @default.
• W1977030002 cites W2063436204 @default.
• W1977030002 cites W2068464983 @default.
• W1977030002 cites W2097536533 @default.
• W1977030002 cites W2102500159 @default.
• W1977030002 cites W2110804359 @default.
• W1977030002 cites W2112811492 @default.
• W1977030002 cites W2112936117 @default.
• W1977030002 cites W2131238404 @default.
• W1977030002 cites W2133488054 @default.
• W1977030002 cites W2144166281 @default.
• W1977030002 cites W2170881245 @default.
• W1977030002 cites W2328438661 @default.
• W1977030002 cites W2466586345 @default.
• W1977030002 doi "https://doi.org/10.1007/s10059-012-2167-7" @default.
• W1977030002 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3887729" @default.
• W1977030002 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22526390" @default.
• W1977030002 hasPublicationYear "2012" @default.
• W1977030002 type Work @default.
• W1977030002 sameAs 1977030002 @default.
• W1977030002 citedByCount "10" @default.
• W1977030002 countsByYear W19770300022014 @default.
• W1977030002 countsByYear W19770300022015 @default.
• W1977030002 countsByYear W19770300022016 @default.
• W1977030002 countsByYear W19770300022017 @default.
• W1977030002 countsByYear W19770300022019 @default.
• W1977030002 crossrefType "journal-article" @default.
• W1977030002 hasAuthorship W1977030002A5001931943 @default.
• W1977030002 hasAuthorship W1977030002A5004021907 @default.
• W1977030002 hasAuthorship W1977030002A5011702150 @default.
• W1977030002 hasAuthorship W1977030002A5014649009 @default.
• W1977030002 hasAuthorship W1977030002A5027062877 @default.
• W1977030002 hasAuthorship W1977030002A5034805413 @default.
• W1977030002 hasAuthorship W1977030002A5044988065 @default.
• W1977030002 hasAuthorship W1977030002A5062342062 @default.
• W1977030002 hasAuthorship W1977030002A5077798472 @default.
• W1977030002 hasBestOaLocation W19770300022 @default.
• W1977030002 hasConcept C104317684 @default.
• W1977030002 hasConcept C12554922 @default.
• W1977030002 hasConcept C15152581 @default.
• W1977030002 hasConcept C153911025 @default.
• W1977030002 hasConcept C159047783 @default.
• W1977030002 hasConcept C16685009 @default.
• W1977030002 hasConcept C185592680 @default.
• W1977030002 hasConcept C203233990 @default.
• W1977030002 hasConcept C28328180 @default.
• W1977030002 hasConcept C32470452 @default.
• W1977030002 hasConcept C40767141 @default.
• W1977030002 hasConcept C54355233 @default.
• W1977030002 hasConcept C71924100 @default.
• W1977030002 hasConcept C81885089 @default.
• W1977030002 hasConcept C86803240 @default.
• W1977030002 hasConcept C95444343 @default.
• W1977030002 hasConceptScore W1977030002C104317684 @default.
• W1977030002 hasConceptScore W1977030002C12554922 @default.
• W1977030002 hasConceptScore W1977030002C15152581 @default.
• W1977030002 hasConceptScore W1977030002C153911025 @default.
• W1977030002 hasConceptScore W1977030002C159047783 @default.
• W1977030002 hasConceptScore W1977030002C16685009 @default.
• W1977030002 hasConceptScore W1977030002C185592680 @default.
• W1977030002 hasConceptScore W1977030002C203233990 @default.
• W1977030002 hasConceptScore W1977030002C28328180 @default.
• W1977030002 hasConceptScore W1977030002C32470452 @default.
• W1977030002 hasConceptScore W1977030002C40767141 @default.
• W1977030002 hasConceptScore W1977030002C54355233 @default.
• W1977030002 hasConceptScore W1977030002C71924100 @default.
• W1977030002 hasConceptScore W1977030002C81885089 @default.
• W1977030002 hasConceptScore W1977030002C86803240 @default.
• W1977030002 hasConceptScore W1977030002C95444343 @default.
• W1977030002 hasIssue "5" @default.
• W1977030002 hasLocation W19770300021 @default.
• W1977030002 hasLocation W19770300022 @default.
• W1977030002 hasLocation W19770300023 @default.
• W1977030002 hasLocation W19770300024 @default.

• next