FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1977057688> ?p ?o ?g. }

• W1977057688 abstract "Abstract Electronic absorption and magnetic circular dichroism (MCD) data are reported for the H175G cavity mutant of yeast cytochrome c peroxidase (H175G CCP) in its ligand-free ferric state at 4 °C under slightly acidic conditions (pH 5.9). Initial analysis of the MCD spectrum of this state suggested a five-coordinate structure with ligation by an aspartate or glutamate. However, examination of the crystal structure of wild-type CCP revealed no plausible carboxylate-containing amino-acid residues close enough to the heme iron to serve as the ligand. An alternative interpretation of the MCD data is that the phosphate ion from the buffer is bound to the ferric heme iron. A phosphate group is similar in electronic character to a carboxylate and could give an MCD signal similar to that of a carboxylate-bound heme. Phosphate coordination to the ferric heme iron, albeit trans to imidazole, has recently been seen in a 2.0-A-resolution crystal structure of imidazole-bound ferric H175G CCP in phosphate buffer [Biochemistry 40 (5) (2001) 1265]. To investigate the validity of phosphate ligation, the pH 5.9 species in the 100 mM phosphate buffer was exchanged into the pH 5.9, 100 mM MES (2-[N-morpholino]ethanesulfonic acid) buffer. The MCD spectrum of the species in the MES buffer was spectrally distinct from that in the phosphate buffer, indicating that the original spectrum depends on the presence of a phosphate ion. We conclude that in the phosphate buffer, the exogenous ligand-free ferric state of the H175G CCP cavity mutant is actually coordinated by a phosphate ion from the buffer." @default.
• W1977057688 created "2016-06-24" @default.
• W1977057688 creator A5002133506 @default.
• W1977057688 creator A5038193276 @default.
• W1977057688 creator A5056046836 @default.
• W1977057688 creator A5078308705 @default.
• W1977057688 date "2002-11-01" @default.
• W1977057688 modified "2023-09-27" @default.
• W1977057688 title "Initial characterization of the ferric H175G cytochrome c peroxidase cavity mutant using magnetic circular dichroism spectroscopy: phosphate from the buffer as an axial ligand" @default.
• W1977057688 cites W1500539090 @default.
• W1977057688 cites W1632575500 @default.
• W1977057688 cites W1970198664 @default.
• W1977057688 cites W1971879307 @default.
• W1977057688 cites W1982406961 @default.
• W1977057688 cites W1993624568 @default.
• W1977057688 cites W2000618159 @default.
• W1977057688 cites W2003092651 @default.
• W1977057688 cites W2006289163 @default.
• W1977057688 cites W2010474135 @default.
• W1977057688 cites W2028801687 @default.
• W1977057688 cites W2031114592 @default.
• W1977057688 cites W2031696049 @default.
• W1977057688 cites W2033740175 @default.
• W1977057688 cites W2034468399 @default.
• W1977057688 cites W2038292842 @default.
• W1977057688 cites W2038648627 @default.
• W1977057688 cites W2041935468 @default.
• W1977057688 cites W2045639174 @default.
• W1977057688 cites W2046824288 @default.
• W1977057688 cites W2069885972 @default.
• W1977057688 cites W2086172313 @default.
• W1977057688 cites W2093683433 @default.
• W1977057688 cites W2094293924 @default.
• W1977057688 cites W2136697302 @default.
• W1977057688 cites W2165898505 @default.
• W1977057688 doi "https://doi.org/10.1016/s0531-5131(02)00150-4" @default.
• W1977057688 hasPublicationYear "2002" @default.
• W1977057688 type Work @default.
• W1977057688 sameAs 1977057688 @default.
• W1977057688 citedByCount "0" @default.
• W1977057688 crossrefType "journal-article" @default.
• W1977057688 hasAuthorship W1977057688A5002133506 @default.
• W1977057688 hasAuthorship W1977057688A5038193276 @default.
• W1977057688 hasAuthorship W1977057688A5056046836 @default.
• W1977057688 hasAuthorship W1977057688A5078308705 @default.
• W1977057688 hasConcept C116569031 @default.
• W1977057688 hasConcept C121332964 @default.
• W1977057688 hasConcept C1276947 @default.
• W1977057688 hasConcept C133166286 @default.
• W1977057688 hasConcept C133571119 @default.
• W1977057688 hasConcept C170493617 @default.
• W1977057688 hasConcept C178790620 @default.
• W1977057688 hasConcept C179104552 @default.
• W1977057688 hasConcept C181199279 @default.
• W1977057688 hasConcept C185592680 @default.
• W1977057688 hasConcept C2776217839 @default.
• W1977057688 hasConcept C2777132085 @default.
• W1977057688 hasConcept C2778074193 @default.
• W1977057688 hasConcept C2778117296 @default.
• W1977057688 hasConcept C2780534640 @default.
• W1977057688 hasConcept C4839761 @default.
• W1977057688 hasConcept C55493867 @default.
• W1977057688 hasConcept C68563686 @default.
• W1977057688 hasConcept C71240020 @default.
• W1977057688 hasConcept C8010536 @default.
• W1977057688 hasConceptScore W1977057688C116569031 @default.
• W1977057688 hasConceptScore W1977057688C121332964 @default.
• W1977057688 hasConceptScore W1977057688C1276947 @default.
• W1977057688 hasConceptScore W1977057688C133166286 @default.
• W1977057688 hasConceptScore W1977057688C133571119 @default.
• W1977057688 hasConceptScore W1977057688C170493617 @default.
• W1977057688 hasConceptScore W1977057688C178790620 @default.
• W1977057688 hasConceptScore W1977057688C179104552 @default.
• W1977057688 hasConceptScore W1977057688C181199279 @default.
• W1977057688 hasConceptScore W1977057688C185592680 @default.
• W1977057688 hasConceptScore W1977057688C2776217839 @default.
• W1977057688 hasConceptScore W1977057688C2777132085 @default.
• W1977057688 hasConceptScore W1977057688C2778074193 @default.
• W1977057688 hasConceptScore W1977057688C2778117296 @default.
• W1977057688 hasConceptScore W1977057688C2780534640 @default.
• W1977057688 hasConceptScore W1977057688C4839761 @default.
• W1977057688 hasConceptScore W1977057688C55493867 @default.
• W1977057688 hasConceptScore W1977057688C68563686 @default.
• W1977057688 hasConceptScore W1977057688C71240020 @default.
• W1977057688 hasConceptScore W1977057688C8010536 @default.
• W1977057688 hasLocation W19770576881 @default.
• W1977057688 hasOpenAccess W1977057688 @default.
• W1977057688 hasPrimaryLocation W19770576881 @default.
• W1977057688 hasRelatedWork W1492269902 @default.
• W1977057688 hasRelatedWork W1777417386 @default.
• W1977057688 hasRelatedWork W1986823072 @default.
• W1977057688 hasRelatedWork W1987835043 @default.
• W1977057688 hasRelatedWork W2073134958 @default.
• W1977057688 hasRelatedWork W2073510310 @default.
• W1977057688 hasRelatedWork W2086678753 @default.
• W1977057688 hasRelatedWork W2286110212 @default.
• W1977057688 hasRelatedWork W2351086876 @default.
• W1977057688 hasRelatedWork W2364463881 @default.
• W1977057688 isParatext "false" @default.
• W1977057688 isRetracted "false" @default.

• next