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- W1977079300 abstract "A combination of reversed-phase chromatographic (RPC) [octadecyl silica (ODS)] and ion-modrated partition chromatographic (IMPC) (Ca2+) stationary phases with water as mobile phase provides separation of the cyanobacterial osmolyte glucosylglycerol (2-O-α-d-glucopyranosylglycerol, GG) from other ubiquitous osmolytes (sucrose, trehalose, glycinebetaine) and major natural carbohydrates, also in the presence of common osmotic stressors (mannitol, sorbitol). The method allows investigations of GG biosynthesis in vitro where glucose and glycerol can be released. The separate use of RPC or IMPC columns is restricted to samples containing no significant amounts of sucrose and glucose, respectively. Amino-bonded silica and acetonitrile-water mixtures provide excellent separation of GG from disaccharides but separation from important hexoses is limited." @default.
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- W1977079300 date "1995-06-01" @default.
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- W1977079300 title "Determination of the cyanobacterial osmolyte glucosylglycerol by high-performance liquid chromatography" @default.
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