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W1977099547 abstract "In the present work, a new reverse phase–high performance liquid chromatography (RP–HPLC) method was developed, validated, and compared for estimation of the bis-triazole antifungal fluconazole (FLZ), in different simulated biological fluids, that is, gastric fluid simulant (GFS, pH = 1.5), vaginal fluid simulant (VFS, pH = 4.2), topical simulated media-phosphate buffer (PB, pH = 6.8), blood plasma simulant-phosphate buffer saline (PBS, pH = 7.4) and in methanol. For RP–HPLC the chromatography was carried out on a octadecyl silane (ODS-3) Hypersil C18 column (250 mm × 4.6 mm × 5 µm) using water (pH 5.2, adjusted with orthophosphoric acid) and acetonitrile (80:20, v/v) as the mobile phase at a flow rate of 2.5 mL/min with detection at 260 nm. The calibration curve of standard FLZ was linear in the range 0.2–40 µg/mL, 0.1–40 µg/L, 0.2–40 µg/mL, 0.1–40 µg/mL, and 0.2–50 µg/mL, in GFS, VFS, PB, PBS, and in methanol, respectively. All the methods were validated with respect to linearity, precision, accuracy, a..." @default.
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W1977099547 date "2013-12-11" @default.
W1977099547 modified "2023-09-27" @default.
W1977099547 title "DEVELOPMENT AND VALIDATION OF NEW HPLC-METHODS FOR ESTIMATION OF FLUCONAZOLE IN DIFFERENT SIMULATED BIOLOGICAL FLUIDS: A COMPARATIVE STUDY" @default.
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W1977099547 doi "https://doi.org/10.1080/10826076.2012.758131" @default.
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