Matches in SemOpenAlex for { <https://semopenalex.org/work/W1977117235> ?p ?o ?g. }
- W1977117235 endingPage "113" @default.
- W1977117235 startingPage "105" @default.
- W1977117235 abstract "Abstract Direct voltammetry of adsorbed redox enzymes at pyrolytic graphite electrodes has shown to be very useful to probe the catalytic activity of several nitrate reductases in the presence of nitrate. In this work we demonstrated that in some cases an electrode-induced haem alteration leads to a loss of nitrate reductase activity. Nitrate reductases are key enzymes in the biological nitrogen cycle. In particular, NapAB from Cupriavidus necator has an important role in the scavenging of nitrate and NarGHI from Marinobacter hydrocarbonoclasticus 617 is essential for the anaerobic respiration. These enzymes present haem groups among their redox centres, which are essential for the electron transfer from the reducing to the oxidising substrate. When adsorbed at graphite electrodes, both enzymes displayed a non-turnover signal corresponding to a one-electron redox process, with formal reduction potentials at pH 7.6 of −159 mV and −139 mV vs. SHE for Nap and Nar, respectively. Both enzymes displayed peroxidase activity at a potential close to that of the non-turnover response. Experiments with the whole enzymes and the haem free NapA from Desulfovibrio desulfuricans and NarGH from M. hydrocarbonoclasticus 617 were a valuable tool to get information about the cofactor undergoing electron transfer. We confirmed that this behaviour is related with the haems present in subunit B and subunit I of NapAB and NarGHI, respectively." @default.
- W1977117235 created "2016-06-24" @default.
- W1977117235 creator A5001383675 @default.
- W1977117235 creator A5002055733 @default.
- W1977117235 creator A5013816962 @default.
- W1977117235 creator A5033437991 @default.
- W1977117235 creator A5080148966 @default.
- W1977117235 creator A5088922007 @default.
- W1977117235 creator A5089637284 @default.
- W1977117235 date "2013-03-01" @default.
- W1977117235 modified "2023-09-25" @default.
- W1977117235 title "Induced peroxidase activity of haem containing nitrate reductases revealed by protein film electrochemistry" @default.
- W1977117235 cites W1491929882 @default.
- W1977117235 cites W1662489613 @default.
- W1977117235 cites W1851503055 @default.
- W1977117235 cites W1889431171 @default.
- W1977117235 cites W1972101693 @default.
- W1977117235 cites W1972795630 @default.
- W1977117235 cites W1975206994 @default.
- W1977117235 cites W1978436100 @default.
- W1977117235 cites W1981183428 @default.
- W1977117235 cites W1982847921 @default.
- W1977117235 cites W1985556019 @default.
- W1977117235 cites W1988833413 @default.
- W1977117235 cites W1992660497 @default.
- W1977117235 cites W1998064253 @default.
- W1977117235 cites W2003280703 @default.
- W1977117235 cites W2009225565 @default.
- W1977117235 cites W2014596582 @default.
- W1977117235 cites W2022668730 @default.
- W1977117235 cites W2027157827 @default.
- W1977117235 cites W2029198060 @default.
- W1977117235 cites W2031215741 @default.
- W1977117235 cites W2033475818 @default.
- W1977117235 cites W2034828882 @default.
- W1977117235 cites W2037064093 @default.
- W1977117235 cites W2042440325 @default.
- W1977117235 cites W2044608755 @default.
- W1977117235 cites W2048649061 @default.
- W1977117235 cites W2052401089 @default.
- W1977117235 cites W2054428479 @default.
- W1977117235 cites W2056287417 @default.
- W1977117235 cites W2057467884 @default.
- W1977117235 cites W2057752367 @default.
- W1977117235 cites W2065542680 @default.
- W1977117235 cites W2065895486 @default.
- W1977117235 cites W2066441404 @default.
- W1977117235 cites W2067813138 @default.
- W1977117235 cites W2086606027 @default.
- W1977117235 cites W2090661426 @default.
- W1977117235 cites W2113879497 @default.
- W1977117235 cites W2118017212 @default.
- W1977117235 cites W2118492712 @default.
- W1977117235 cites W2140616203 @default.
- W1977117235 cites W2950584898 @default.
- W1977117235 cites W4237049974 @default.
- W1977117235 cites W4245399940 @default.
- W1977117235 doi "https://doi.org/10.1016/j.jelechem.2013.01.030" @default.
- W1977117235 hasPublicationYear "2013" @default.
- W1977117235 type Work @default.
- W1977117235 sameAs 1977117235 @default.
- W1977117235 citedByCount "6" @default.
- W1977117235 countsByYear W19771172352013 @default.
- W1977117235 countsByYear W19771172352014 @default.
- W1977117235 countsByYear W19771172352017 @default.
- W1977117235 countsByYear W19771172352020 @default.
- W1977117235 countsByYear W19771172352021 @default.
- W1977117235 crossrefType "journal-article" @default.
- W1977117235 hasAuthorship W1977117235A5001383675 @default.
- W1977117235 hasAuthorship W1977117235A5002055733 @default.
- W1977117235 hasAuthorship W1977117235A5013816962 @default.
- W1977117235 hasAuthorship W1977117235A5033437991 @default.
- W1977117235 hasAuthorship W1977117235A5080148966 @default.
- W1977117235 hasAuthorship W1977117235A5088922007 @default.
- W1977117235 hasAuthorship W1977117235A5089637284 @default.
- W1977117235 hasConcept C13965031 @default.
- W1977117235 hasConcept C147789679 @default.
- W1977117235 hasConcept C162008176 @default.
- W1977117235 hasConcept C17525397 @default.
- W1977117235 hasConcept C178790620 @default.
- W1977117235 hasConcept C179104552 @default.
- W1977117235 hasConcept C181199279 @default.
- W1977117235 hasConcept C185592680 @default.
- W1977117235 hasConcept C20705724 @default.
- W1977117235 hasConcept C2776217839 @default.
- W1977117235 hasConcept C2776384668 @default.
- W1977117235 hasConcept C2778710519 @default.
- W1977117235 hasConcept C52859227 @default.
- W1977117235 hasConcept C55493867 @default.
- W1977117235 hasConceptScore W1977117235C13965031 @default.
- W1977117235 hasConceptScore W1977117235C147789679 @default.
- W1977117235 hasConceptScore W1977117235C162008176 @default.
- W1977117235 hasConceptScore W1977117235C17525397 @default.
- W1977117235 hasConceptScore W1977117235C178790620 @default.
- W1977117235 hasConceptScore W1977117235C179104552 @default.
- W1977117235 hasConceptScore W1977117235C181199279 @default.
- W1977117235 hasConceptScore W1977117235C185592680 @default.
- W1977117235 hasConceptScore W1977117235C20705724 @default.