FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1977122905> ?p ?o ?g. }

• W1977122905 endingPage "102" @default.
• W1977122905 startingPage "93" @default.
• W1977122905 abstract "The Embryonic Stem cell Test (EST) is an in vitro alternative test designed for the prediction of embryotoxicity. The endpoint of the test is the interference with mesoderm-derived cardiac muscle differentiation observed under the microscope as beating muscle foci. The relative subjectivity of this endpoint, as well as the applicability domain and related predictivity need further to be defined to facilitate implementation of the EST into regulatory strategies. The use of transcriptomics techniques to monitor differentiation-related gene expression changes in the EST might improve the EST in each of these aspects. Therefore, we studied the gene expression profile in embryonic stem cells (ESC) in the early phase of differentiation and its modulation by exposure to the well known embryotoxicant monobutyl phthalate (MBP). Cells were exposed from the early embryoid body stage onwards and RNA was collected after 6, 12 and 24 h of exposure. Samples were hybridized to spotted microarrays, containing 21,997-mer oligonucleotides. Differential gene expression patterns were analyzed. A total number of 43 genes that were found to be upregulated in this study as a consequence of induction of cardiomyocyte differentiation were combined in a gene set, named ‘VAN_DARTEL_HEARTDIFF_24H’. Gene Set Enrichment Analysis (GSEA) comparative analysis using multiple gene set collections clearly showed that temporal changes in gene expression were functionally related to cardiomyocyte differentiation. Furthermore, exposure of embryoid bodies (EB) to MBP increased expression of pluripotency-, proliferation- and nonmesodermal differentiation-related gene sets, which indicates inhibition of mesodermal differentiation. The inhibition of mesoderm-derived cardiomyocyte differentiation by MBP exposure was most obvious through the downregulation of our novel gene set identified in this study, ‘VAN_DARTEL_HEARTDIFF_24H’, which specifically describes the niche of early cardiomyocyte differentiation. The gene set defined in this study might serve as a starting point for defining a dedicated gene set for early detection of embryotoxicity in the EST. Such a gene set may serve as an improved endpoint in the EST as compared to morphology, and will allow a more detailed definition of the applicability domain and predictivity of EST." @default.
• W1977122905 created "2016-06-24" @default.
• W1977122905 creator A5010620767 @default.
• W1977122905 creator A5024589017 @default.
• W1977122905 creator A5038697595 @default.
• W1977122905 creator A5071796957 @default.
• W1977122905 creator A5073820178 @default.
• W1977122905 date "2009-04-01" @default.
• W1977122905 modified "2023-10-16" @default.
• W1977122905 title "Early gene expression changes during embryonic stem cell differentiation into cardiomyocytes and their modulation by monobutyl phthalate" @default.
• W1977122905 cites W1948938001 @default.
• W1977122905 cites W1965428318 @default.
• W1977122905 cites W1978683007 @default.
• W1977122905 cites W1981814090 @default.
• W1977122905 cites W1986477473 @default.
• W1977122905 cites W1990711550 @default.
• W1977122905 cites W1995969645 @default.
• W1977122905 cites W2001433883 @default.
• W1977122905 cites W2002686737 @default.
• W1977122905 cites W2012777643 @default.
• W1977122905 cites W2024133736 @default.
• W1977122905 cites W2032111322 @default.
• W1977122905 cites W2044985097 @default.
• W1977122905 cites W2052031890 @default.
• W1977122905 cites W2083320231 @default.
• W1977122905 cites W2088994822 @default.
• W1977122905 cites W2089285124 @default.
• W1977122905 cites W2092912085 @default.
• W1977122905 cites W2104611151 @default.
• W1977122905 cites W2105712639 @default.
• W1977122905 cites W2106840146 @default.
• W1977122905 cites W2112723752 @default.
• W1977122905 cites W2122070996 @default.
• W1977122905 cites W2122683221 @default.
• W1977122905 cites W2124711114 @default.
• W1977122905 cites W2130410032 @default.
• W1977122905 cites W2132556212 @default.
• W1977122905 cites W2145172444 @default.
• W1977122905 cites W2150048579 @default.
• W1977122905 cites W2159675211 @default.
• W1977122905 cites W2162696534 @default.
• W1977122905 cites W2164683872 @default.
• W1977122905 cites W2165764675 @default.
• W1977122905 cites W4255149674 @default.
• W1977122905 doi "https://doi.org/10.1016/j.reprotox.2008.12.009" @default.
• W1977122905 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19162170" @default.
• W1977122905 hasPublicationYear "2009" @default.
• W1977122905 type Work @default.
• W1977122905 sameAs 1977122905 @default.
• W1977122905 citedByCount "69" @default.
• W1977122905 countsByYear W19771229052012 @default.
• W1977122905 countsByYear W19771229052013 @default.
• W1977122905 countsByYear W19771229052014 @default.
• W1977122905 countsByYear W19771229052015 @default.
• W1977122905 countsByYear W19771229052016 @default.
• W1977122905 countsByYear W19771229052017 @default.
• W1977122905 countsByYear W19771229052018 @default.
• W1977122905 countsByYear W19771229052019 @default.
• W1977122905 countsByYear W19771229052020 @default.
• W1977122905 countsByYear W19771229052022 @default.
• W1977122905 crossrefType "journal-article" @default.
• W1977122905 hasAuthorship W1977122905A5010620767 @default.
• W1977122905 hasAuthorship W1977122905A5024589017 @default.
• W1977122905 hasAuthorship W1977122905A5038697595 @default.
• W1977122905 hasAuthorship W1977122905A5071796957 @default.
• W1977122905 hasAuthorship W1977122905A5073820178 @default.
• W1977122905 hasConcept C104317684 @default.
• W1977122905 hasConcept C107459253 @default.
• W1977122905 hasConcept C143948831 @default.
• W1977122905 hasConcept C145103041 @default.
• W1977122905 hasConcept C148738053 @default.
• W1977122905 hasConcept C150194340 @default.
• W1977122905 hasConcept C153911025 @default.
• W1977122905 hasConcept C162317418 @default.
• W1977122905 hasConcept C165864922 @default.
• W1977122905 hasConcept C2780915607 @default.
• W1977122905 hasConcept C54355233 @default.
• W1977122905 hasConcept C86803240 @default.
• W1977122905 hasConcept C95444343 @default.
• W1977122905 hasConceptScore W1977122905C104317684 @default.
• W1977122905 hasConceptScore W1977122905C107459253 @default.
• W1977122905 hasConceptScore W1977122905C143948831 @default.
• W1977122905 hasConceptScore W1977122905C145103041 @default.
• W1977122905 hasConceptScore W1977122905C148738053 @default.
• W1977122905 hasConceptScore W1977122905C150194340 @default.
• W1977122905 hasConceptScore W1977122905C153911025 @default.
• W1977122905 hasConceptScore W1977122905C162317418 @default.
• W1977122905 hasConceptScore W1977122905C165864922 @default.
• W1977122905 hasConceptScore W1977122905C2780915607 @default.
• W1977122905 hasConceptScore W1977122905C54355233 @default.
• W1977122905 hasConceptScore W1977122905C86803240 @default.
• W1977122905 hasConceptScore W1977122905C95444343 @default.
• W1977122905 hasIssue "2" @default.
• W1977122905 hasLocation W19771229051 @default.
• W1977122905 hasLocation W19771229052 @default.
• W1977122905 hasOpenAccess W1977122905 @default.
• W1977122905 hasPrimaryLocation W19771229051 @default.
• W1977122905 hasRelatedWork W145559274 @default.

• next