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- W1977132401 endingPage "606" @default.
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- W1977132401 abstract "The affinity-labeling technique is an extremely important method in receptor biochemistry. The 3-nitro-2-pyridinesulfenyl (Npys) group, attached to a mercapto group, can react only with a free thiol group (the beta-mercapto group of cysteine residue) of the target receptor molecules, forming a disulfide bond. This disulfide bonding is mediated through the thiol-disulfide exchange reaction. Unlike other labeling methods, the approach utilizing such chemically activated thiol-containing ligands is able to reproduce an unlabeled protein by treatment with dithiothreitol, a reducing reagent. This provides several unique aspects for the studies elucidating the structure-function relationships between the peptide and the receptor. Based on the SNpys affinity technique, we have achieved the discriminative disulfide-bonding affinity labeling of the three different subtypes of opioid receptors: mu, delta and kappa. This article reviews our novel affinity techniques in the in vitro receptor biochemistry." @default.
- W1977132401 created "2016-06-24" @default.
- W1977132401 creator A5007893586 @default.
- W1977132401 creator A5042570402 @default.
- W1977132401 date "2001-10-01" @default.
- W1977132401 modified "2023-10-16" @default.
- W1977132401 title "Discriminative disulfide-bonding affinity labeling of opioid receptor subtypes" @default.
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- W1977132401 doi "https://doi.org/10.1016/s0165-022x(01)00222-6" @default.
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