Matches in SemOpenAlex for { <https://semopenalex.org/work/W1977249314> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W1977249314 abstract "In recent years, artificial nanometer-sized pores (nanopores) have been successfully employed as a new tool to detect the passage of DNA molecules. When a molecule is driven through the nanopore by an externally applied electric field, it blocks part of the ionic current, resulting in a temporal current blockade. So far, only translocations of bare DNA have been reported. In this work, we extend this to protein-coated DNA molecules by including the well-studied RecA protein, which plays an essential role in the central steps of homologous recombination. To accomplish its DNA strand exchange activities, RecA polymerizes onto DNA to form a stiff helical nucleoprotein filament. These filaments translocate through solid-state pores, as we demonstrate unambiguously. Additionally, we use the nanopore system in conjunction with an integrated optical tweezer, which allows us to insert the RecA-DNA complex into the nanopore. We will discuss results as they pertain to both the charge structure and conformation of the complex. This work also lays the groundwork for future experiments on sequential screening of proteins, say transcription factors, which locally coat DNA." @default.
- W1977249314 created "2016-06-24" @default.
- W1977249314 creator A5034623724 @default.
- W1977249314 creator A5050833977 @default.
- W1977249314 creator A5060960924 @default.
- W1977249314 creator A5066937642 @default.
- W1977249314 creator A5089996499 @default.
- W1977249314 date "2009-02-01" @default.
- W1977249314 modified "2023-09-29" @default.
- W1977249314 title "Static and Dynamic Investigations of RecA-DNA Complex with a Solid-State Nanopore" @default.
- W1977249314 doi "https://doi.org/10.1016/j.bpj.2008.12.3858" @default.
- W1977249314 hasPublicationYear "2009" @default.
- W1977249314 type Work @default.
- W1977249314 sameAs 1977249314 @default.
- W1977249314 citedByCount "0" @default.
- W1977249314 crossrefType "journal-article" @default.
- W1977249314 hasAuthorship W1977249314A5034623724 @default.
- W1977249314 hasAuthorship W1977249314A5050833977 @default.
- W1977249314 hasAuthorship W1977249314A5060960924 @default.
- W1977249314 hasAuthorship W1977249314A5066937642 @default.
- W1977249314 hasAuthorship W1977249314A5089996499 @default.
- W1977249314 hasBestOaLocation W19772493141 @default.
- W1977249314 hasConcept C102744134 @default.
- W1977249314 hasConcept C12554922 @default.
- W1977249314 hasConcept C126513998 @default.
- W1977249314 hasConcept C141795571 @default.
- W1977249314 hasConcept C14228908 @default.
- W1977249314 hasConcept C145148216 @default.
- W1977249314 hasConcept C159467904 @default.
- W1977249314 hasConcept C171250308 @default.
- W1977249314 hasConcept C178790620 @default.
- W1977249314 hasConcept C185592680 @default.
- W1977249314 hasConcept C191961535 @default.
- W1977249314 hasConcept C192562407 @default.
- W1977249314 hasConcept C2182769 @default.
- W1977249314 hasConcept C32909587 @default.
- W1977249314 hasConcept C51679486 @default.
- W1977249314 hasConcept C552990157 @default.
- W1977249314 hasConcept C55493867 @default.
- W1977249314 hasConcept C60325843 @default.
- W1977249314 hasConcept C86803240 @default.
- W1977249314 hasConceptScore W1977249314C102744134 @default.
- W1977249314 hasConceptScore W1977249314C12554922 @default.
- W1977249314 hasConceptScore W1977249314C126513998 @default.
- W1977249314 hasConceptScore W1977249314C141795571 @default.
- W1977249314 hasConceptScore W1977249314C14228908 @default.
- W1977249314 hasConceptScore W1977249314C145148216 @default.
- W1977249314 hasConceptScore W1977249314C159467904 @default.
- W1977249314 hasConceptScore W1977249314C171250308 @default.
- W1977249314 hasConceptScore W1977249314C178790620 @default.
- W1977249314 hasConceptScore W1977249314C185592680 @default.
- W1977249314 hasConceptScore W1977249314C191961535 @default.
- W1977249314 hasConceptScore W1977249314C192562407 @default.
- W1977249314 hasConceptScore W1977249314C2182769 @default.
- W1977249314 hasConceptScore W1977249314C32909587 @default.
- W1977249314 hasConceptScore W1977249314C51679486 @default.
- W1977249314 hasConceptScore W1977249314C552990157 @default.
- W1977249314 hasConceptScore W1977249314C55493867 @default.
- W1977249314 hasConceptScore W1977249314C60325843 @default.
- W1977249314 hasConceptScore W1977249314C86803240 @default.
- W1977249314 hasLocation W19772493141 @default.
- W1977249314 hasOpenAccess W1977249314 @default.
- W1977249314 hasPrimaryLocation W19772493141 @default.
- W1977249314 hasRelatedWork W1980621375 @default.
- W1977249314 hasRelatedWork W1987612842 @default.
- W1977249314 hasRelatedWork W2038505879 @default.
- W1977249314 hasRelatedWork W2064928172 @default.
- W1977249314 hasRelatedWork W2070849241 @default.
- W1977249314 hasRelatedWork W2071754291 @default.
- W1977249314 hasRelatedWork W2084517368 @default.
- W1977249314 hasRelatedWork W2127856514 @default.
- W1977249314 hasRelatedWork W2567360045 @default.
- W1977249314 hasRelatedWork W2581690074 @default.
- W1977249314 hasRelatedWork W2751045871 @default.
- W1977249314 hasRelatedWork W2765160869 @default.
- W1977249314 hasRelatedWork W2765161614 @default.
- W1977249314 hasRelatedWork W2775471907 @default.
- W1977249314 hasRelatedWork W2790125256 @default.
- W1977249314 hasRelatedWork W2950357632 @default.
- W1977249314 hasRelatedWork W3137931250 @default.
- W1977249314 hasRelatedWork W3148274165 @default.
- W1977249314 hasRelatedWork W3152637380 @default.
- W1977249314 hasRelatedWork W956999217 @default.
- W1977249314 isParatext "false" @default.
- W1977249314 isRetracted "false" @default.
- W1977249314 magId "1977249314" @default.
- W1977249314 workType "article" @default.