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• W1977278451 abstract "Chitosan was chosen as a natural polymer for dispersion of single walled carbon nanotubes (SWNT) based on its ability to efficiently solubilize SWNTs to form a stable dispersion. Moreover, chitosan films deposited on a surface of a glassy carbon (GC) electrode are mechanically stable. Further stabilisation of the chitosan film containing SWNT (CHIT–SWNT) was done by chemical crosslinking with glutaraldehyde and free aldehyde groups produced a substrate used for covalent immobilisation of galactose oxidase (GalOD). Different galactose biosensor configurations were tested with optimisation of composition of inner and outer membrane; and enzyme immobilisation procedure, as well. Detection of oxygen uptake by GalOD on CHIT–SWNT layer at −400 mV is robust and, when flow injection analysis (FIA) was applied for assays, a low detection limit (25 μM) and very high assay throughput rate (150 h−1) was achieved. This new galactose biosensor offers highly reliable detection of galactose with R.S.D. well below 2% and it has been successfully applied to assaying galactose in a blood sample with recovery index between 101.2 and 102.7%." @default.
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• W1977278451 date "2007-03-15" @default.
• W1977278451 modified "2023-10-17" @default.
• W1977278451 title "The use of single walled carbon nanotubes dispersed in a chitosan matrix for preparation of a galactose biosensor" @default.
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• W1977278451 doi "https://doi.org/10.1016/j.bios.2006.08.014" @default.
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