Matches in SemOpenAlex for { <https://semopenalex.org/work/W1977279455> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W1977279455 endingPage "12982" @default.
- W1977279455 startingPage "12973" @default.
- W1977279455 abstract "The Azotobacter vinelandii FeSII protein confers conformational protection to nitrogenase by binding to the MoFe and Fe proteins under periods of oxidative stress to create an inactive but O2-stabilized tripartite complex. In this work the FeSII protein has been overexpressed in Escherichia coli, and the recombinant protein has been purified to homogeneity, crystallized, and characterized in terms of its functional, spectroscopic, and redox properties. The recombinant protein is a homodimer and is expressed as a holoprotein with one [2Fe-2S]2+,+ cluster in each subunit. It is shown to be functional in reconstituting an O2-stable nitrogenase complex in vitro. Spectroscopic studies using the combination of UV-visible absorption, CD, and variable temperature MCD, EPR, and resonance Raman indicate that the [2Fe-2S]2+,+ cluster is coordinated exclusively by cysteine residues. The arrangement of coordinating cysteines in the primary sequence and the EPR properties of the [2Fe-2S]+ cluster (g = 2.04, 1.95, 1.88) are very similar to those of chloroplast ferredoxins. However, the variable-temperature MCD, resonance Raman, and redox properties (Em = -262 +/- 10 mV based on dye-mediated EPR redox titrations) are more characteristic of hydroxylase-type ferredoxins such as adrenodoxin. In contrast to chloroplast-type ferredoxins, the vibrational properties of the [2Fe-2S]2+,+ cluster in the FeSII protein indicate that none of the cysteinyl Fe-S-C-C dihedral angles are close to 180 degrees and that the cluster is not exposed to solvent. Preliminary X-ray diffraction analysis indicates that the protein crystallizes in an orthorhombic space group with unit cell dimensions a = 135 A, b = 135 A, and c = 38 A and that there are at least two dimers per asymmetric unit." @default.
- W1977279455 created "2016-06-24" @default.
- W1977279455 creator A5021602538 @default.
- W1977279455 creator A5040226815 @default.
- W1977279455 creator A5074496781 @default.
- W1977279455 creator A5076187405 @default.
- W1977279455 date "1995-10-10" @default.
- W1977279455 modified "2023-09-25" @default.
- W1977279455 title "The nitrogenase-protective FeSII protein of Azotobacter vinelandii: overexpression, characterization, and crystallization" @default.
- W1977279455 doi "https://doi.org/10.1021/bi00040a007" @default.
- W1977279455 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7548055" @default.
- W1977279455 hasPublicationYear "1995" @default.
- W1977279455 type Work @default.
- W1977279455 sameAs 1977279455 @default.
- W1977279455 citedByCount "30" @default.
- W1977279455 countsByYear W19772794552012 @default.
- W1977279455 countsByYear W19772794552014 @default.
- W1977279455 countsByYear W19772794552015 @default.
- W1977279455 countsByYear W19772794552016 @default.
- W1977279455 countsByYear W19772794552017 @default.
- W1977279455 countsByYear W19772794552018 @default.
- W1977279455 countsByYear W19772794552019 @default.
- W1977279455 countsByYear W19772794552020 @default.
- W1977279455 countsByYear W19772794552021 @default.
- W1977279455 countsByYear W19772794552022 @default.
- W1977279455 countsByYear W19772794552023 @default.
- W1977279455 crossrefType "journal-article" @default.
- W1977279455 hasAuthorship W1977279455A5021602538 @default.
- W1977279455 hasAuthorship W1977279455A5040226815 @default.
- W1977279455 hasAuthorship W1977279455A5074496781 @default.
- W1977279455 hasAuthorship W1977279455A5076187405 @default.
- W1977279455 hasConcept C121332964 @default.
- W1977279455 hasConcept C134621786 @default.
- W1977279455 hasConcept C141280058 @default.
- W1977279455 hasConcept C178790620 @default.
- W1977279455 hasConcept C179104552 @default.
- W1977279455 hasConcept C181199279 @default.
- W1977279455 hasConcept C181440489 @default.
- W1977279455 hasConcept C185592680 @default.
- W1977279455 hasConcept C187961010 @default.
- W1977279455 hasConcept C2776159390 @default.
- W1977279455 hasConcept C2777315085 @default.
- W1977279455 hasConcept C2777852564 @default.
- W1977279455 hasConcept C2779201268 @default.
- W1977279455 hasConcept C36786875 @default.
- W1977279455 hasConcept C46141821 @default.
- W1977279455 hasConcept C537208039 @default.
- W1977279455 hasConcept C55493867 @default.
- W1977279455 hasConcept C55904794 @default.
- W1977279455 hasConcept C8010536 @default.
- W1977279455 hasConceptScore W1977279455C121332964 @default.
- W1977279455 hasConceptScore W1977279455C134621786 @default.
- W1977279455 hasConceptScore W1977279455C141280058 @default.
- W1977279455 hasConceptScore W1977279455C178790620 @default.
- W1977279455 hasConceptScore W1977279455C179104552 @default.
- W1977279455 hasConceptScore W1977279455C181199279 @default.
- W1977279455 hasConceptScore W1977279455C181440489 @default.
- W1977279455 hasConceptScore W1977279455C185592680 @default.
- W1977279455 hasConceptScore W1977279455C187961010 @default.
- W1977279455 hasConceptScore W1977279455C2776159390 @default.
- W1977279455 hasConceptScore W1977279455C2777315085 @default.
- W1977279455 hasConceptScore W1977279455C2777852564 @default.
- W1977279455 hasConceptScore W1977279455C2779201268 @default.
- W1977279455 hasConceptScore W1977279455C36786875 @default.
- W1977279455 hasConceptScore W1977279455C46141821 @default.
- W1977279455 hasConceptScore W1977279455C537208039 @default.
- W1977279455 hasConceptScore W1977279455C55493867 @default.
- W1977279455 hasConceptScore W1977279455C55904794 @default.
- W1977279455 hasConceptScore W1977279455C8010536 @default.
- W1977279455 hasIssue "40" @default.
- W1977279455 hasLocation W19772794551 @default.
- W1977279455 hasLocation W19772794552 @default.
- W1977279455 hasOpenAccess W1977279455 @default.
- W1977279455 hasPrimaryLocation W19772794551 @default.
- W1977279455 hasRelatedWork W1974086171 @default.
- W1977279455 hasRelatedWork W1977279455 @default.
- W1977279455 hasRelatedWork W1997352785 @default.
- W1977279455 hasRelatedWork W2036779219 @default.
- W1977279455 hasRelatedWork W2068688630 @default.
- W1977279455 hasRelatedWork W2083701649 @default.
- W1977279455 hasRelatedWork W2342664984 @default.
- W1977279455 hasRelatedWork W2478334354 @default.
- W1977279455 hasRelatedWork W4214602348 @default.
- W1977279455 hasRelatedWork W4214628847 @default.
- W1977279455 hasVolume "34" @default.
- W1977279455 isParatext "false" @default.
- W1977279455 isRetracted "false" @default.
- W1977279455 magId "1977279455" @default.
- W1977279455 workType "article" @default.