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- W1977286534 abstract "Abstract Under certain reaction conditions formaldehyde induced strong fluorescence with tryptophyl-peptides on silica gel thin layer permitting the detection of submicrogram amounts. Microspectrofluorometric analysis revealed that the spectral properties of the fluorophores were identical for the various tryptophyl-peptides but varied with the reaction conditions and sometimes with the concentration of the compound. At neutral pH maximum emission was at about 500 mμ, a value that was only moderately affected by the concentration. With combined formaldehyde-HCl treatment maximum emission was markedly concentration-dependent: 460 mμ at low concentrations, 550 mμ at high concentrations. The spectral properties of the tryptophan fluorophore differed markedly from those of the peptide fluorophores after treatment with gaseous and aqueous formaldehyde but not after formaldehyde-HCl. Solvent systems for the separation of tryptophyl-peptides are described." @default.
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- W1977286534 date "1972-04-01" @default.
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- W1977286534 title "Separation of tryptophyl-peptides by thin-layer chromatography and microspectrofluorometric characterization of their formaldehyde-induced fluorophores" @default.
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- W1977286534 doi "https://doi.org/10.1016/0003-2697(72)90321-1" @default.
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