Matches in SemOpenAlex for { <https://semopenalex.org/work/W1977314241> ?p ?o ?g. }
- W1977314241 endingPage "460" @default.
- W1977314241 startingPage "453" @default.
- W1977314241 abstract "The objective of this study is to investigate the effect of intracellular photosensitizer distribution on tumor cell death after photodynamic therapy (PDT).The photosensitizer accumulates in tumor tissue during PDT, and generates intracellular reactive oxygen species (ROS), resulting in tumor cell death.This study was carried out to elucidate the effects of PDT in a KB oral cancer cell line using hematoporphyrin with irradiation at 635 nm and 5 mW/cm(2). After irradiation, the MTT reduction method, agarose gel electrophoresis, flow cytometry, and Diff-Quick staining were performed. The intracellular ROS level was measured by DCF-DA. Intracellular hematoporphyrin was monitored with a confocal microscope, and Western blot and caspase activity assays were performed.In our study, cell survival was reduced by about 50% after 3 h of hematoporphyrin incubation time. In DNA fragmentation, flow cytometry, and Diff-Quick assay, necrosis was identified within 12 h and apoptosis soon thereafter. Confocal microscopy revealed that hematoporphyrin was localized in the cell membrane, cytoplasm, and nucleus as time passed. The quantities of intracellular ROS correlated with the time of hematoporphyrin accumulation. Additionally, Western blot analysis of Bcl-2/Bax, the release of cytochrome C, and activity of caspase-3 and caspase-9 showed that apoptosis followed the mitochondria-dependent pathway.PDT with hematoporphyrin in the KB cell line showed morphological changes of cell necrosis and apoptosis, which were associated with the time of distribution and localization of hematoporphyrin. Also, the apoptosis evoked followed the mitochondria-dependent pathway." @default.
- W1977314241 created "2016-06-24" @default.
- W1977314241 creator A5000358579 @default.
- W1977314241 creator A5009122002 @default.
- W1977314241 creator A5029438050 @default.
- W1977314241 creator A5038217592 @default.
- W1977314241 creator A5041533546 @default.
- W1977314241 creator A5045118797 @default.
- W1977314241 creator A5050155068 @default.
- W1977314241 creator A5058973542 @default.
- W1977314241 creator A5059629287 @default.
- W1977314241 creator A5060167939 @default.
- W1977314241 creator A5085160385 @default.
- W1977314241 creator A5088399826 @default.
- W1977314241 date "2009-06-01" @default.
- W1977314241 modified "2023-10-02" @default.
- W1977314241 title "Cell Death and Intracellular Distribution of Hematoporphyrin in a KB Cell Line" @default.
- W1977314241 cites W168674480 @default.
- W1977314241 cites W1916661321 @default.
- W1977314241 cites W1942214720 @default.
- W1977314241 cites W1971292240 @default.
- W1977314241 cites W1988272953 @default.
- W1977314241 cites W2022830981 @default.
- W1977314241 cites W2022874289 @default.
- W1977314241 cites W2030257965 @default.
- W1977314241 cites W2034564919 @default.
- W1977314241 cites W2040269973 @default.
- W1977314241 cites W2042309736 @default.
- W1977314241 cites W2053406437 @default.
- W1977314241 cites W2058452428 @default.
- W1977314241 cites W2064810566 @default.
- W1977314241 cites W2090020685 @default.
- W1977314241 cites W2097717323 @default.
- W1977314241 cites W2107672277 @default.
- W1977314241 cites W2111645003 @default.
- W1977314241 cites W2116791229 @default.
- W1977314241 cites W2129339346 @default.
- W1977314241 cites W2145073891 @default.
- W1977314241 cites W2152501814 @default.
- W1977314241 cites W2166648549 @default.
- W1977314241 cites W2241139458 @default.
- W1977314241 cites W2397954396 @default.
- W1977314241 cites W4211024586 @default.
- W1977314241 doi "https://doi.org/10.1089/pho.2008.2334" @default.
- W1977314241 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19416004" @default.
- W1977314241 hasPublicationYear "2009" @default.
- W1977314241 type Work @default.
- W1977314241 sameAs 1977314241 @default.
- W1977314241 citedByCount "17" @default.
- W1977314241 countsByYear W19773142412012 @default.
- W1977314241 countsByYear W19773142412014 @default.
- W1977314241 countsByYear W19773142412015 @default.
- W1977314241 countsByYear W19773142412016 @default.
- W1977314241 countsByYear W19773142412017 @default.
- W1977314241 countsByYear W19773142412018 @default.
- W1977314241 countsByYear W19773142412019 @default.
- W1977314241 countsByYear W19773142412020 @default.
- W1977314241 countsByYear W19773142412022 @default.
- W1977314241 crossrefType "journal-article" @default.
- W1977314241 hasAuthorship W1977314241A5000358579 @default.
- W1977314241 hasAuthorship W1977314241A5009122002 @default.
- W1977314241 hasAuthorship W1977314241A5029438050 @default.
- W1977314241 hasAuthorship W1977314241A5038217592 @default.
- W1977314241 hasAuthorship W1977314241A5041533546 @default.
- W1977314241 hasAuthorship W1977314241A5045118797 @default.
- W1977314241 hasAuthorship W1977314241A5050155068 @default.
- W1977314241 hasAuthorship W1977314241A5058973542 @default.
- W1977314241 hasAuthorship W1977314241A5059629287 @default.
- W1977314241 hasAuthorship W1977314241A5060167939 @default.
- W1977314241 hasAuthorship W1977314241A5085160385 @default.
- W1977314241 hasAuthorship W1977314241A5088399826 @default.
- W1977314241 hasConcept C153911025 @default.
- W1977314241 hasConcept C178790620 @default.
- W1977314241 hasConcept C185592680 @default.
- W1977314241 hasConcept C190283241 @default.
- W1977314241 hasConcept C2780699678 @default.
- W1977314241 hasConcept C2781323092 @default.
- W1977314241 hasConcept C2781469039 @default.
- W1977314241 hasConcept C29311851 @default.
- W1977314241 hasConcept C31573885 @default.
- W1977314241 hasConcept C553184892 @default.
- W1977314241 hasConcept C55493867 @default.
- W1977314241 hasConcept C79879829 @default.
- W1977314241 hasConcept C86803240 @default.
- W1977314241 hasConcept C95444343 @default.
- W1977314241 hasConceptScore W1977314241C153911025 @default.
- W1977314241 hasConceptScore W1977314241C178790620 @default.
- W1977314241 hasConceptScore W1977314241C185592680 @default.
- W1977314241 hasConceptScore W1977314241C190283241 @default.
- W1977314241 hasConceptScore W1977314241C2780699678 @default.
- W1977314241 hasConceptScore W1977314241C2781323092 @default.
- W1977314241 hasConceptScore W1977314241C2781469039 @default.
- W1977314241 hasConceptScore W1977314241C29311851 @default.
- W1977314241 hasConceptScore W1977314241C31573885 @default.
- W1977314241 hasConceptScore W1977314241C553184892 @default.
- W1977314241 hasConceptScore W1977314241C55493867 @default.
- W1977314241 hasConceptScore W1977314241C79879829 @default.
- W1977314241 hasConceptScore W1977314241C86803240 @default.