FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1977315244> ?p ?o ?g. }

• W1977315244 endingPage "e110127" @default.
• W1977315244 startingPage "e110127" @default.
• W1977315244 abstract "Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) despite being increasingly used as a method for microbial identification, still present limitations in which concerns the differentiation of closely related species. Bacillus pumillus and Bacillus safensis, are species of biotechnological and pharmaceutical significance, difficult to differentiate by conventional methodologies. In this study, using a well-characterized collection of B. pumillus and B. safensis isolates, we demonstrated the suitability of MALDI-TOF-MS combined with chemometrics to accurately and rapidly identify them. Moreover, characteristic species-specific ion masses were tentatively assigned, using UniProtKB/Swiss-Prot and UniProtKB/TrEMBL databases and primary literature. Delineation of B. pumilus (ions at m/z 5271 and 6122) and B. safensis (ions at m/z 5288, 5568 and 6413) species were supported by a congruent characteristic protein pattern. Moreover, using a chemometric approach, the score plot created by partial least square discriminant analysis (PLSDA) of mass spectra demonstrated the presence of two individualized clusters, each one enclosing isolates belonging to a species-specific spectral group. The generated pool of species-specific proteins comprised mostly ribosomal and SASPs proteins. Therefore, in B. pumilus the specific ion at m/z 5271 was associated with a small acid-soluble spore protein (SASP O) or with 50S protein L35, whereas in B. safensis specific ions at m/z 5288 and 5568 were associated with SASP J and P, respectively, and an ion at m/z 6413 with 50S protein L32. Thus, the resulting unique protein profile combined with chemometric analysis, proved to be valuable tools for B. pumilus and B. safensis discrimination, allowing their reliable, reproducible and rapid identification." @default.
• W1977315244 created "2016-06-24" @default.
• W1977315244 creator A5002919691 @default.
• W1977315244 creator A5035142978 @default.
• W1977315244 creator A5041192920 @default.
• W1977315244 creator A5050780648 @default.
• W1977315244 creator A5071001364 @default.
• W1977315244 creator A5073498288 @default.
• W1977315244 date "2014-10-14" @default.
• W1977315244 modified "2023-10-01" @default.
• W1977315244 title "Differentiation of Bacillus pumilus and Bacillus safensis Using MALDI-TOF-MS" @default.
• W1977315244 cites W1839847945 @default.
• W1977315244 cites W1904976200 @default.
• W1977315244 cites W1964354371 @default.
• W1977315244 cites W1965493275 @default.
• W1977315244 cites W1978391662 @default.
• W1977315244 cites W1982950807 @default.
• W1977315244 cites W1985376812 @default.
• W1977315244 cites W1994772145 @default.
• W1977315244 cites W1997270584 @default.
• W1977315244 cites W1997383178 @default.
• W1977315244 cites W2027297527 @default.
• W1977315244 cites W2038811930 @default.
• W1977315244 cites W2049386269 @default.
• W1977315244 cites W2080375516 @default.
• W1977315244 cites W2089046621 @default.
• W1977315244 cites W2094594436 @default.
• W1977315244 cites W2114980859 @default.
• W1977315244 cites W2117350727 @default.
• W1977315244 cites W2119982021 @default.
• W1977315244 cites W2124981918 @default.
• W1977315244 cites W2129222620 @default.
• W1977315244 cites W2130755203 @default.
• W1977315244 cites W2131693321 @default.
• W1977315244 cites W2131950604 @default.
• W1977315244 cites W2140698273 @default.
• W1977315244 cites W2155860425 @default.
• W1977315244 cites W2156116033 @default.
• W1977315244 cites W2156977043 @default.
• W1977315244 cites W2160278600 @default.
• W1977315244 cites W2163514057 @default.
• W1977315244 cites W2164583936 @default.
• W1977315244 cites W2165587270 @default.
• W1977315244 cites W2171125527 @default.
• W1977315244 cites W746605148 @default.
• W1977315244 doi "https://doi.org/10.1371/journal.pone.0110127" @default.
• W1977315244 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4196992" @default.
• W1977315244 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25314655" @default.
• W1977315244 hasPublicationYear "2014" @default.
• W1977315244 type Work @default.
• W1977315244 sameAs 1977315244 @default.
• W1977315244 citedByCount "40" @default.
• W1977315244 countsByYear W19773152442015 @default.
• W1977315244 countsByYear W19773152442016 @default.
• W1977315244 countsByYear W19773152442017 @default.
• W1977315244 countsByYear W19773152442018 @default.
• W1977315244 countsByYear W19773152442019 @default.
• W1977315244 countsByYear W19773152442020 @default.
• W1977315244 countsByYear W19773152442021 @default.
• W1977315244 countsByYear W19773152442022 @default.
• W1977315244 countsByYear W19773152442023 @default.
• W1977315244 crossrefType "journal-article" @default.
• W1977315244 hasAuthorship W1977315244A5002919691 @default.
• W1977315244 hasAuthorship W1977315244A5035142978 @default.
• W1977315244 hasAuthorship W1977315244A5041192920 @default.
• W1977315244 hasAuthorship W1977315244A5050780648 @default.
• W1977315244 hasAuthorship W1977315244A5071001364 @default.
• W1977315244 hasAuthorship W1977315244A5073498288 @default.
• W1977315244 hasBestOaLocation W19773152441 @default.
• W1977315244 hasConcept C104317684 @default.
• W1977315244 hasConcept C150394285 @default.
• W1977315244 hasConcept C162356407 @default.
• W1977315244 hasConcept C162711632 @default.
• W1977315244 hasConcept C178790620 @default.
• W1977315244 hasConcept C185592680 @default.
• W1977315244 hasConcept C202264299 @default.
• W1977315244 hasConcept C2778187402 @default.
• W1977315244 hasConcept C43617362 @default.
• W1977315244 hasConcept C523546767 @default.
• W1977315244 hasConcept C54355233 @default.
• W1977315244 hasConcept C55493867 @default.
• W1977315244 hasConcept C70721500 @default.
• W1977315244 hasConcept C75280812 @default.
• W1977315244 hasConcept C86803240 @default.
• W1977315244 hasConceptScore W1977315244C104317684 @default.
• W1977315244 hasConceptScore W1977315244C150394285 @default.
• W1977315244 hasConceptScore W1977315244C162356407 @default.
• W1977315244 hasConceptScore W1977315244C162711632 @default.
• W1977315244 hasConceptScore W1977315244C178790620 @default.
• W1977315244 hasConceptScore W1977315244C185592680 @default.
• W1977315244 hasConceptScore W1977315244C202264299 @default.
• W1977315244 hasConceptScore W1977315244C2778187402 @default.
• W1977315244 hasConceptScore W1977315244C43617362 @default.
• W1977315244 hasConceptScore W1977315244C523546767 @default.
• W1977315244 hasConceptScore W1977315244C54355233 @default.
• W1977315244 hasConceptScore W1977315244C55493867 @default.
• W1977315244 hasConceptScore W1977315244C70721500 @default.
• W1977315244 hasConceptScore W1977315244C75280812 @default.

• next