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- W1977319522 abstract "Kindlins are essential coactivators, with talin, of the cell surface receptors integrins and also participate in integrin outside-in signalling, and the control of gene transcription in the cell nucleus. The kindlins are ~75 kDa multidomain proteins and bind to an NPxY motif and upstream T/S cluster of the integrin β-subunit cytoplasmic tail. The hematopoietically-important kindlin isoform, kindlin-3, is critical for platelet aggregation during thrombus formation, leukocyte rolling in response to infection and inflammation and osteoclast podocyte formation in bone resorption. Kindlin-3's role in these processes has resulted in extensive cellular and physiological studies. However, there is a need for an efficient method of acquiring high quality milligram quantities of the protein for further studies. We have developed a protocol, here described, for the efficient expression and purification of recombinant murine kindlin-3 by use of a baculovirus-driven expression system in Sf9 cells yielding sufficient amounts of high purity full-length protein to allow its biophysical characterization. The same approach could be taken in the study of the other mammalian kindlin isoforms." @default.
- W1977319522 created "2016-06-24" @default.
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- W1977319522 date "2014-03-19" @default.
- W1977319522 modified "2023-09-29" @default.
- W1977319522 title "Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies" @default.
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- W1977319522 doi "https://doi.org/10.3791/51206" @default.
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