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- W1977322250 abstract "We developed a sensitive method for detecting adenosine monophosphate (AMP) by using polyphosphate (polyP)–AMP phosphotransferase (PPT) and adenylate kinase (ADK) from Acinetobacter johnsonii in conjugation with firefly luciferase. This method allowed us to detect AMP over the concentration range of 0.3–300 pmol per assay. Samples taken from various sources susceptible to bacterial contamination were subjected to AMP assay using the present method because AMP is known to have potential for use as a reliable indicator in hygiene monitoring. The results showed that the present method was able to detect food residues with high sensitivity. The present method was also proved to be useful for the quantification of RNA. After digesting RNA with nuclease S1, the present method could detect RNA at concentrations as low as 600 pg." @default.
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- W1977322250 date "2001-12-01" @default.
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- W1977322250 title "A sensitive method for detecting AMP by utilizing polyphosphate-dependent ATP regeneration and bioluminescence reactions" @default.
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- W1977322250 doi "https://doi.org/10.1016/s1369-703x(01)00144-9" @default.
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