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- W1977492738 abstract "Single cells from suspension cultures of Datura innoxia (Mill.) and mesophyll protoplasts of Nicotiana tabacum (L.) cv. xanthi were cultured individually in 10–25 nl microdroplets of synthetic and unconditioned culture medium under a thin layer of mineral oil. More than 50 % of individually cultivated single cells of Datura innoxia (Mill.) survived for more than two weeks, divisions were found in more than 30 % of the cells, callus formation occured at a frequency of up to 30 %. Buffering of media is strictly required for individual culture of single cells under our conditions. The pH of the culture medium has a more pronounced influence on the frequency of callus formation than on the proportions of surviving cells and cells undergoing one division only. More than 40 % of individually cultivated mesophyll protoplats of Nicotiana tabacum (L.) cv. xanthi survive for more than two weeks. Divisions and callus formation occur at frequencies of more than 30 %, and 30 %, respectively." @default.
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- W1977492738 date "1983-09-01" @default.
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- W1977492738 title "Individual Culture of Selected Single Cells and Protoplasts of Higher Plants in Microdroplets of Defined Media" @default.
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- W1977492738 doi "https://doi.org/10.1016/s0044-328x(83)80059-2" @default.
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