FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1977515181> ?p ?o ?g. }

• W1977515181 endingPage "166" @default.
• W1977515181 startingPage "159" @default.
• W1977515181 abstract "Here we report a new isotopic pair of derivatization reagents, ω-bromoacetonylquinolinium bromide (BQB) and d7-ω-bromoacetonylquinolinium bromide (d7-BQB). BQB and d7-BQB both rapidly and selectively reacted with thiols in acidic medium within 3 min with the aid of a microwave. Reduced thiols and total thiols in urine were labeled with BQB and d7-BQB, respectively. The BQB- and d7-BQB-labeled urine samples were then mixed and separated on a HILIC (hydrophilic interaction chromatography) column followed by electrospray ionization tandem mass spectrometry (ESI–MS/MS) detection. The new strategy, which we have named isotope differential derivatization, allows us to simultaneously determine thiols and oxidized thiols in a single run. Compared with positive mode ESI detection of unlabeled thiols, the positive mode ESI–MS signal intensities of BQB-labeled thiols were found to increase by 10-, 20-, and 40-fold for cysteine (Cys), homocysteine (HCys), and glutathione (GSH), respectively (unlabeled N-acetylcysteine (Nac) is difficult to detect by ESI–MS in positive mode due to its low ionization efficiency). The detection limits calculated at a signal-to-noise ratio of 3 were found to be 8.02, 1.56, 0.833, and 3.27 nmol/L for Cys, HCys, Nac, and GSH, respectively. Recoveries of thiols and disulfides from spiked urine samples were between 80% and 105%. The method was successfully used to determine thiols and oxidized thiols in urine samples of 25 healthy volunteers." @default.
• W1977515181 created "2016-06-24" @default.
• W1977515181 creator A5031172482 @default.
• W1977515181 creator A5037432321 @default.
• W1977515181 creator A5040331037 @default.
• W1977515181 creator A5045960607 @default.
• W1977515181 creator A5046130704 @default.
• W1977515181 date "2011-09-01" @default.
• W1977515181 modified "2023-10-12" @default.
• W1977515181 title "Use of isotope differential derivatization for simultaneous determination of thiols and oxidized thiols by liquid chromatography tandem mass spectrometry" @default.
• W1977515181 cites W1964911695 @default.
• W1977515181 cites W1965274678 @default.
• W1977515181 cites W1970310293 @default.
• W1977515181 cites W1976738948 @default.
• W1977515181 cites W1980837820 @default.
• W1977515181 cites W1983266711 @default.
• W1977515181 cites W1983919959 @default.
• W1977515181 cites W1989155308 @default.
• W1977515181 cites W1992377286 @default.
• W1977515181 cites W1998060148 @default.
• W1977515181 cites W2012879076 @default.
• W1977515181 cites W2013742341 @default.
• W1977515181 cites W2019312107 @default.
• W1977515181 cites W2020925489 @default.
• W1977515181 cites W2021122140 @default.
• W1977515181 cites W2028403283 @default.
• W1977515181 cites W2028784365 @default.
• W1977515181 cites W2037948102 @default.
• W1977515181 cites W2038756569 @default.
• W1977515181 cites W2041245259 @default.
• W1977515181 cites W2052046770 @default.
• W1977515181 cites W2055769555 @default.
• W1977515181 cites W2058144487 @default.
• W1977515181 cites W2060253214 @default.
• W1977515181 cites W2062669701 @default.
• W1977515181 cites W2065070740 @default.
• W1977515181 cites W2068479712 @default.
• W1977515181 cites W2070382674 @default.
• W1977515181 cites W2075758080 @default.
• W1977515181 cites W2079492156 @default.
• W1977515181 cites W2088026100 @default.
• W1977515181 cites W2093879208 @default.
• W1977515181 cites W2104717580 @default.
• W1977515181 cites W2116567644 @default.
• W1977515181 cites W2132878122 @default.
• W1977515181 cites W2139180343 @default.
• W1977515181 cites W2140695271 @default.
• W1977515181 cites W2151682472 @default.
• W1977515181 cites W2316413781 @default.
• W1977515181 cites W4245887746 @default.
• W1977515181 doi "https://doi.org/10.1016/j.ab.2011.05.020" @default.
• W1977515181 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21672511" @default.
• W1977515181 hasPublicationYear "2011" @default.
• W1977515181 type Work @default.
• W1977515181 sameAs 1977515181 @default.
• W1977515181 citedByCount "70" @default.
• W1977515181 countsByYear W19775151812012 @default.
• W1977515181 countsByYear W19775151812013 @default.
• W1977515181 countsByYear W19775151812014 @default.
• W1977515181 countsByYear W19775151812015 @default.
• W1977515181 countsByYear W19775151812016 @default.
• W1977515181 countsByYear W19775151812017 @default.
• W1977515181 countsByYear W19775151812018 @default.
• W1977515181 countsByYear W19775151812019 @default.
• W1977515181 countsByYear W19775151812020 @default.
• W1977515181 countsByYear W19775151812021 @default.
• W1977515181 countsByYear W19775151812022 @default.
• W1977515181 countsByYear W19775151812023 @default.
• W1977515181 crossrefType "journal-article" @default.
• W1977515181 hasAuthorship W1977515181A5031172482 @default.
• W1977515181 hasAuthorship W1977515181A5037432321 @default.
• W1977515181 hasAuthorship W1977515181A5040331037 @default.
• W1977515181 hasAuthorship W1977515181A5045960607 @default.
• W1977515181 hasAuthorship W1977515181A5046130704 @default.
• W1977515181 hasConcept C116817701 @default.
• W1977515181 hasConcept C119128265 @default.
• W1977515181 hasConcept C162356407 @default.
• W1977515181 hasConcept C178790620 @default.
• W1977515181 hasConcept C179998833 @default.
• W1977515181 hasConcept C181199279 @default.
• W1977515181 hasConcept C185592680 @default.
• W1977515181 hasConcept C2776300020 @default.
• W1977515181 hasConcept C2776923573 @default.
• W1977515181 hasConcept C2780026642 @default.
• W1977515181 hasConcept C2781324293 @default.
• W1977515181 hasConcept C31827203 @default.
• W1977515181 hasConcept C40684141 @default.
• W1977515181 hasConcept C40875361 @default.
• W1977515181 hasConcept C43617362 @default.
• W1977515181 hasConcept C538909803 @default.
• W1977515181 hasConcept C55493867 @default.
• W1977515181 hasConceptScore W1977515181C116817701 @default.
• W1977515181 hasConceptScore W1977515181C119128265 @default.
• W1977515181 hasConceptScore W1977515181C162356407 @default.
• W1977515181 hasConceptScore W1977515181C178790620 @default.
• W1977515181 hasConceptScore W1977515181C179998833 @default.
• W1977515181 hasConceptScore W1977515181C181199279 @default.
• W1977515181 hasConceptScore W1977515181C185592680 @default.

• next