FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1977521766> ?p ?o ?g. }

• W1977521766 abstract "The enzyme hypoxanthine-guanine phosphoribosyltransferase (HGPRT) of Plasmodium falciparum plays a key role in the salvage of preformed purine nucleotides from parasite-infected erythrocytes. Since P. falciparum cannot synthesize purines de novo, development of inhibitors specific for the parasite HGPRT should be an effective method of chemotherapy. To provide sufficient amounts of HGPRT for biochemical and crystallographic analysis, we have isolated the P. falciparum HPRT cDNA sequence and expressed it in an Escherichia coli strain deficient for both de novo purine synthesis and guanine utilization (strain GP120). GP120 cells containing the P. falciparum HPRT plasmid vector (pRD500), when grown in the presence of isopropyl-beta-D-thiogalactopyranoside (IPTG) which induces the tac promoter of the expression vector, produce a novel protein of 26 kDa, which is in agreement with the predicted Mr deduced from the HPRT cDNA open reading frame. In addition, we have demonstrated significant HGPRT activity in cell-free extracts of GP120[pRD500] cultures grown in minimal medium containing xanthine, as the sole source of purines, and IPTG." @default.
• W1977521766 created "2016-06-24" @default.
• W1977521766 creator A5001979226 @default.
• W1977521766 creator A5018804463 @default.
• W1977521766 creator A5045874910 @default.
• W1977521766 creator A5063585096 @default.
• W1977521766 date "1990-01-01" @default.
• W1977521766 modified "2023-09-23" @default.
• W1977521766 title "Cloning and expression in Escherichia coli of a hypoxanthine-guanine phosphoribosyltransferase-encoding cDNA from Plasmodium falciparum" @default.
• W1977521766 cites W1963709212 @default.
• W1977521766 cites W1973395514 @default.
• W1977521766 cites W1981253302 @default.
• W1977521766 cites W2002141635 @default.
• W1977521766 cites W2013817482 @default.
• W1977521766 cites W2019864695 @default.
• W1977521766 cites W2023033821 @default.
• W1977521766 cites W2035965789 @default.
• W1977521766 cites W2040381235 @default.
• W1977521766 cites W2051992969 @default.
• W1977521766 cites W2064417483 @default.
• W1977521766 cites W2069841722 @default.
• W1977521766 cites W2082685909 @default.
• W1977521766 cites W2083649056 @default.
• W1977521766 cites W2093807640 @default.
• W1977521766 cites W2100837269 @default.
• W1977521766 cites W2105105392 @default.
• W1977521766 cites W2132897827 @default.
• W1977521766 cites W2138270253 @default.
• W1977521766 cites W2144634347 @default.
• W1977521766 cites W2396040381 @default.
• W1977521766 cites W2414450500 @default.
• W1977521766 cites W2983676549 @default.
• W1977521766 doi "https://doi.org/10.1016/0378-1119(90)90163-l" @default.
• W1977521766 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2205541" @default.
• W1977521766 hasPublicationYear "1990" @default.
• W1977521766 type Work @default.
• W1977521766 sameAs 1977521766 @default.
• W1977521766 citedByCount "21" @default.
• W1977521766 countsByYear W19775217662014 @default.
• W1977521766 countsByYear W19775217662020 @default.
• W1977521766 crossrefType "journal-article" @default.
• W1977521766 hasAuthorship W1977521766A5001979226 @default.
• W1977521766 hasAuthorship W1977521766A5018804463 @default.
• W1977521766 hasAuthorship W1977521766A5045874910 @default.
• W1977521766 hasAuthorship W1977521766A5063585096 @default.
• W1977521766 hasConcept C104317684 @default.
• W1977521766 hasConcept C143065580 @default.
• W1977521766 hasConcept C153911025 @default.
• W1977521766 hasConcept C167625842 @default.
• W1977521766 hasConcept C170344550 @default.
• W1977521766 hasConcept C181199279 @default.
• W1977521766 hasConcept C187882448 @default.
• W1977521766 hasConcept C203014093 @default.
• W1977521766 hasConcept C22744801 @default.
• W1977521766 hasConcept C2777991568 @default.
• W1977521766 hasConcept C2778048844 @default.
• W1977521766 hasConcept C2778301229 @default.
• W1977521766 hasConcept C2778371730 @default.
• W1977521766 hasConcept C2781116151 @default.
• W1977521766 hasConcept C2909697302 @default.
• W1977521766 hasConcept C33161422 @default.
• W1977521766 hasConcept C40767141 @default.
• W1977521766 hasConcept C47289529 @default.
• W1977521766 hasConcept C512185932 @default.
• W1977521766 hasConcept C547475151 @default.
• W1977521766 hasConcept C552990157 @default.
• W1977521766 hasConcept C55493867 @default.
• W1977521766 hasConcept C78179603 @default.
• W1977521766 hasConcept C86803240 @default.
• W1977521766 hasConceptScore W1977521766C104317684 @default.
• W1977521766 hasConceptScore W1977521766C143065580 @default.
• W1977521766 hasConceptScore W1977521766C153911025 @default.
• W1977521766 hasConceptScore W1977521766C167625842 @default.
• W1977521766 hasConceptScore W1977521766C170344550 @default.
• W1977521766 hasConceptScore W1977521766C181199279 @default.
• W1977521766 hasConceptScore W1977521766C187882448 @default.
• W1977521766 hasConceptScore W1977521766C203014093 @default.
• W1977521766 hasConceptScore W1977521766C22744801 @default.
• W1977521766 hasConceptScore W1977521766C2777991568 @default.
• W1977521766 hasConceptScore W1977521766C2778048844 @default.
• W1977521766 hasConceptScore W1977521766C2778301229 @default.
• W1977521766 hasConceptScore W1977521766C2778371730 @default.
• W1977521766 hasConceptScore W1977521766C2781116151 @default.
• W1977521766 hasConceptScore W1977521766C2909697302 @default.
• W1977521766 hasConceptScore W1977521766C33161422 @default.
• W1977521766 hasConceptScore W1977521766C40767141 @default.
• W1977521766 hasConceptScore W1977521766C47289529 @default.
• W1977521766 hasConceptScore W1977521766C512185932 @default.
• W1977521766 hasConceptScore W1977521766C547475151 @default.
• W1977521766 hasConceptScore W1977521766C552990157 @default.
• W1977521766 hasConceptScore W1977521766C55493867 @default.
• W1977521766 hasConceptScore W1977521766C78179603 @default.
• W1977521766 hasConceptScore W1977521766C86803240 @default.
• W1977521766 hasLocation W19775217661 @default.
• W1977521766 hasLocation W19775217662 @default.
• W1977521766 hasOpenAccess W1977521766 @default.
• W1977521766 hasPrimaryLocation W19775217661 @default.
• W1977521766 hasRelatedWork W1594569184 @default.
• W1977521766 hasRelatedWork W1630992622 @default.
• W1977521766 hasRelatedWork W1977521766 @default.

• next