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- W1977584229 abstract "Syringomycin (SR) production by Pseudomonas syringae in potato dextrose broth (PDB) still culture was approximately 20 to 136 times greater than that in potato dextrose agar (PDA) or in PDB shake cultures, respectively. Potato broth supplemented with 1·5% dextrose and 0·4% casamino acids was best for SR production when compared to other formulations of PDB, synthetic medium or biphasic media. Maximum production of SR corresponded with exponential cell growth of P. syringae in vitro. SR activity was completely partitioned into n-butanol from aqueous concentrates of acidified cultures. By means of ion-exchange chromatography (carboxy-methyl cellulose), thin-layer chromatography and Polyacrylamide gel electrophoresis, homogeneous preparations of SR were obtained with specific activities up to 47·9 units/μg. Eleven strains of P. syringae antibiotic toward Geotrichum candidum and representing a broad range of ecotypes were compared for the production of SR or other antibiotics. The only antibiotic isolated from ten of the strains was SR whereas the eleventh strain produced only syringotoxin (ST), a small biocidal polypeptide. Both SR and ST were phytotoxic and mimicked holcus spot on maize leaves at a threshold concentration of 50 units/ml. The threshold toxigenicity of SR to representative isolates of P. syringae ranged from 800 to 6400 units/ml compared with 100 units/ml for G. candidum." @default.
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- W1977584229 date "1977-07-01" @default.
- W1977584229 modified "2023-09-25" @default.
- W1977584229 title "Production and purification of syringomycin, a phytotoxin produced by Pseudomonas syringae" @default.
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- W1977584229 doi "https://doi.org/10.1016/0048-4059(77)90083-2" @default.
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