FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1977615108> ?p ?o ?g. }

• W1977615108 endingPage "4419" @default.
• W1977615108 startingPage "4419" @default.
• W1977615108 abstract "The ability of neurons to extend projections and to form physical connections among them (i.e., “connect-ability”) is altered in several neuropathologies. The quantification of these alterations is an important read-out to investigate pathogenic mechanisms and for research and development of neuropharmacological therapies, however current morphological analysis methods are very time-intensive. Here, we present and characterize a novel on-chip approach that we propose as a rapid assay. Our approach is based on the definition on a neuronal cell culture substrate of discrete patterns of adhesion protein spots (poly-D-lysine, 23 ± 5 μm in diameter) characterized by controlled inter-spot separations of increasing distance (from 40 μm to 100 μm), locally adsorbed in an adhesion-repulsive agarose layer. Under these conditions, the connect-ability of wild type primary neurons from rodents is shown to be strictly dependent on the inter-spot distance, and can be rapidly documented by simple optical read-outs. Moreover, we applied our approach to identify connect-ability defects in neurons from a mouse model of 22q11.2 deletion syndrome/DiGeorge syndrome, by comparative trials with wild type preparations. The presented results demonstrate the sensitivity and reliability of this novel on-chip-based connect-ability approach and validate the use of this method for the rapid assessment of neuronal connect-ability defects in neuropathologies." @default.
• W1977615108 created "2016-06-24" @default.
• W1977615108 creator A5019538304 @default.
• W1977615108 creator A5022921649 @default.
• W1977615108 creator A5053398823 @default.
• W1977615108 creator A5082004546 @default.
• W1977615108 creator A5087177497 @default.
• W1977615108 creator A5088983421 @default.
• W1977615108 date "2013-01-01" @default.
• W1977615108 modified "2023-10-16" @default.
• W1977615108 title "Nano-volume drop patterning for rapid on-chip neuronal connect-ability assays" @default.
• W1977615108 cites W1971635936 @default.
• W1977615108 cites W1977608305 @default.
• W1977615108 cites W1989752993 @default.
• W1977615108 cites W1992009091 @default.
• W1977615108 cites W1992063064 @default.
• W1977615108 cites W1999761643 @default.
• W1977615108 cites W2000541034 @default.
• W1977615108 cites W2003304872 @default.
• W1977615108 cites W2005484961 @default.
• W1977615108 cites W2011541551 @default.
• W1977615108 cites W2021258666 @default.
• W1977615108 cites W2024925941 @default.
• W1977615108 cites W2027735953 @default.
• W1977615108 cites W2035814108 @default.
• W1977615108 cites W2047451107 @default.
• W1977615108 cites W2047975465 @default.
• W1977615108 cites W2048260860 @default.
• W1977615108 cites W2056046435 @default.
• W1977615108 cites W2065781758 @default.
• W1977615108 cites W2065943966 @default.
• W1977615108 cites W2067074069 @default.
• W1977615108 cites W2068707928 @default.
• W1977615108 cites W2081697226 @default.
• W1977615108 cites W2081747437 @default.
• W1977615108 cites W2082114896 @default.
• W1977615108 cites W2086037466 @default.
• W1977615108 cites W2086373463 @default.
• W1977615108 cites W2087035953 @default.
• W1977615108 cites W2093510885 @default.
• W1977615108 cites W2094520929 @default.
• W1977615108 cites W2095119525 @default.
• W1977615108 cites W2095455882 @default.
• W1977615108 cites W2095491050 @default.
• W1977615108 cites W2098173227 @default.
• W1977615108 cites W2102407454 @default.
• W1977615108 cites W2104738142 @default.
• W1977615108 cites W2104953138 @default.
• W1977615108 cites W2105503408 @default.
• W1977615108 cites W2109591733 @default.
• W1977615108 cites W2110133891 @default.
• W1977615108 cites W2110674840 @default.
• W1977615108 cites W2115720575 @default.
• W1977615108 cites W2140279570 @default.
• W1977615108 cites W2159500608 @default.
• W1977615108 cites W2161220967 @default.
• W1977615108 cites W2161754293 @default.
• W1977615108 cites W2466319129 @default.
• W1977615108 cites W4249587697 @default.
• W1977615108 doi "https://doi.org/10.1039/c3lc50564b" @default.
• W1977615108 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24064674" @default.
• W1977615108 hasPublicationYear "2013" @default.
• W1977615108 type Work @default.
• W1977615108 sameAs 1977615108 @default.
• W1977615108 citedByCount "21" @default.
• W1977615108 countsByYear W19776151082014 @default.
• W1977615108 countsByYear W19776151082015 @default.
• W1977615108 countsByYear W19776151082016 @default.
• W1977615108 countsByYear W19776151082018 @default.
• W1977615108 countsByYear W19776151082019 @default.
• W1977615108 countsByYear W19776151082020 @default.
• W1977615108 countsByYear W19776151082021 @default.
• W1977615108 crossrefType "journal-article" @default.
• W1977615108 hasAuthorship W1977615108A5019538304 @default.
• W1977615108 hasAuthorship W1977615108A5022921649 @default.
• W1977615108 hasAuthorship W1977615108A5053398823 @default.
• W1977615108 hasAuthorship W1977615108A5082004546 @default.
• W1977615108 hasAuthorship W1977615108A5087177497 @default.
• W1977615108 hasAuthorship W1977615108A5088983421 @default.
• W1977615108 hasConcept C153911025 @default.
• W1977615108 hasConcept C169760540 @default.
• W1977615108 hasConcept C171250308 @default.
• W1977615108 hasConcept C185592680 @default.
• W1977615108 hasConcept C192562407 @default.
• W1977615108 hasConcept C2778476535 @default.
• W1977615108 hasConcept C41008148 @default.
• W1977615108 hasConcept C70721500 @default.
• W1977615108 hasConcept C86803240 @default.
• W1977615108 hasConcept C95444343 @default.
• W1977615108 hasConceptScore W1977615108C153911025 @default.
• W1977615108 hasConceptScore W1977615108C169760540 @default.
• W1977615108 hasConceptScore W1977615108C171250308 @default.
• W1977615108 hasConceptScore W1977615108C185592680 @default.
• W1977615108 hasConceptScore W1977615108C192562407 @default.
• W1977615108 hasConceptScore W1977615108C2778476535 @default.
• W1977615108 hasConceptScore W1977615108C41008148 @default.
• W1977615108 hasConceptScore W1977615108C70721500 @default.
• W1977615108 hasConceptScore W1977615108C86803240 @default.

• next