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• W1977623218 endingPage "167" @default.
• W1977623218 startingPage "147" @default.
• W1977623218 abstract "Alternating current glow discharge in vapors of amylamine renders carbon films suitable for electron microscope preparations of nucleic acids and proteins. The molecules are evenly distributed on the film and deposited with an efficiency of 10–20 %. Length measurements on naked, uncoated DNA (circular replicative form of fd and λ phages) demonstrate the reproducible results obtained with the method. Preliminary observations on RNA polymerase attached to DNA suggest that the internal structures of the protein may be observed and that the enzyme is probably attached asymmetrically to the DNA. The high contrast of dark-field microscopy permits resolution of biological structure at the limit of the microscope. Other factors, like background structures of the carbon film (microcrystallites) and destruction of the object, now become limiting factors." @default.
• W1977623218 created "2016-06-24" @default.
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• W1977623218 date "1971-04-01" @default.
• W1977623218 modified "2023-09-23" @default.
• W1977623218 title "A new preparation method for dark-field electron microscopy of biomacromolecules" @default.
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• W1977623218 doi "https://doi.org/10.1016/s0022-5320(71)80148-x" @default.
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