FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1977706989> ?p ?o ?g. }

• W1977706989 endingPage "1685" @default.
• W1977706989 startingPage "1677" @default.
• W1977706989 abstract "There is increasing evidence to implicate a role for CD8(+) T cells in protective immunity against tuberculosis. Recombinant vaccinia (rVV) expressing Mycobacterium tuberculosis (MTB) proteins can be used both as tools to dissect CD8(+) T-cell responses and, in attenuated form, as candidate vaccines capable of inducing a balanced CD4(+)/CD8(+) T-cell response. A panel of rVV was constructed to express four immunodominant secreted proteins of MTB: 85A, 85B and 85C and ESAT-6. A parallel group of rVV was constructed to include the heterologous eukaryotic tissue plasminogen activator (tPA) signal sequence to assess if this would enhance expression and immunogenicity. Clear expression was obtained for 85A, 85B and ESAT-6 and the addition of tPA resulted in N-glycosylation and a 4-10-fold increase in expression. Female C57BL/6 mice were immunised using the rVV-Ag85 constructs, and interleukin-2 and gamma-interferon were assayed using a co-culture of immune splenocytes and recall antigen. There was a marked increase in cytokine production in mice immunised with the tPA-containing constructs. We report the first data demonstrating enhanced immunogenicity of rVV using a tPA signal sequence, which has significant implications for future vaccine design." @default.
• W1977706989 created "2016-06-24" @default.
• W1977706989 creator A5014608759 @default.
• W1977706989 creator A5015913342 @default.
• W1977706989 creator A5043338123 @default.
• W1977706989 creator A5045879589 @default.
• W1977706989 creator A5057970065 @default.
• W1977706989 creator A5089522455 @default.
• W1977706989 creator A5090428914 @default.
• W1977706989 date "2000-11-01" @default.
• W1977706989 modified "2023-10-14" @default.
• W1977706989 title "Vaccinia expression of Mycobacterium tuberculosis-secreted proteins: tissue plasminogen activator signal sequence enhances expression and immunogenicity of M. tuberculosis Ag85" @default.
• W1977706989 cites W1521353535 @default.
• W1977706989 cites W1801265254 @default.
• W1977706989 cites W1877277475 @default.
• W1977706989 cites W1912689844 @default.
• W1977706989 cites W1912825022 @default.
• W1977706989 cites W1915468037 @default.
• W1977706989 cites W1915893748 @default.
• W1977706989 cites W1975045527 @default.
• W1977706989 cites W1991933020 @default.
• W1977706989 cites W1995317461 @default.
• W1977706989 cites W2004604338 @default.
• W1977706989 cites W2004764321 @default.
• W1977706989 cites W2005059536 @default.
• W1977706989 cites W2008209529 @default.
• W1977706989 cites W2012228399 @default.
• W1977706989 cites W2015855242 @default.
• W1977706989 cites W2016336432 @default.
• W1977706989 cites W2053951102 @default.
• W1977706989 cites W2059349344 @default.
• W1977706989 cites W2062860313 @default.
• W1977706989 cites W2065704868 @default.
• W1977706989 cites W2069762663 @default.
• W1977706989 cites W2086662036 @default.
• W1977706989 cites W2086940218 @default.
• W1977706989 cites W2090260968 @default.
• W1977706989 cites W2100894095 @default.
• W1977706989 cites W2102122618 @default.
• W1977706989 cites W2111354206 @default.
• W1977706989 cites W2114104820 @default.
• W1977706989 cites W2118905950 @default.
• W1977706989 cites W2126225865 @default.
• W1977706989 cites W2134990166 @default.
• W1977706989 cites W2139497847 @default.
• W1977706989 cites W2141714831 @default.
• W1977706989 cites W2142021677 @default.
• W1977706989 cites W2145504015 @default.
• W1977706989 cites W2157605618 @default.
• W1977706989 cites W2157656284 @default.
• W1977706989 cites W2158035277 @default.
• W1977706989 cites W2158204548 @default.
• W1977706989 cites W2166347165 @default.
• W1977706989 cites W2166582198 @default.
• W1977706989 cites W2418150095 @default.
• W1977706989 cites W4313347805 @default.
• W1977706989 doi "https://doi.org/10.1016/s1286-4579(00)01323-x" @default.
• W1977706989 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11137041" @default.
• W1977706989 hasPublicationYear "2000" @default.
• W1977706989 type Work @default.
• W1977706989 sameAs 1977706989 @default.
• W1977706989 citedByCount "26" @default.
• W1977706989 countsByYear W19777069892012 @default.
• W1977706989 countsByYear W19777069892013 @default.
• W1977706989 countsByYear W19777069892014 @default.
• W1977706989 countsByYear W19777069892018 @default.
• W1977706989 countsByYear W19777069892019 @default.
• W1977706989 countsByYear W19777069892020 @default.
• W1977706989 countsByYear W19777069892023 @default.
• W1977706989 crossrefType "journal-article" @default.
• W1977706989 hasAuthorship W1977706989A5014608759 @default.
• W1977706989 hasAuthorship W1977706989A5015913342 @default.
• W1977706989 hasAuthorship W1977706989A5043338123 @default.
• W1977706989 hasAuthorship W1977706989A5045879589 @default.
• W1977706989 hasAuthorship W1977706989A5057970065 @default.
• W1977706989 hasAuthorship W1977706989A5089522455 @default.
• W1977706989 hasAuthorship W1977706989A5090428914 @default.
• W1977706989 hasConcept C142724271 @default.
• W1977706989 hasConcept C159047783 @default.
• W1977706989 hasConcept C167672396 @default.
• W1977706989 hasConcept C203014093 @default.
• W1977706989 hasConcept C2777975735 @default.
• W1977706989 hasConcept C2779053233 @default.
• W1977706989 hasConcept C2780868878 @default.
• W1977706989 hasConcept C2781069245 @default.
• W1977706989 hasConcept C71924100 @default.
• W1977706989 hasConcept C86803240 @default.
• W1977706989 hasConcept C8891405 @default.
• W1977706989 hasConceptScore W1977706989C142724271 @default.
• W1977706989 hasConceptScore W1977706989C159047783 @default.
• W1977706989 hasConceptScore W1977706989C167672396 @default.
• W1977706989 hasConceptScore W1977706989C203014093 @default.
• W1977706989 hasConceptScore W1977706989C2777975735 @default.
• W1977706989 hasConceptScore W1977706989C2779053233 @default.
• W1977706989 hasConceptScore W1977706989C2780868878 @default.
• W1977706989 hasConceptScore W1977706989C2781069245 @default.
• W1977706989 hasConceptScore W1977706989C71924100 @default.
• W1977706989 hasConceptScore W1977706989C86803240 @default.

• next