FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1977711214> ?p ?o ?g. }

• W1977711214 endingPage "S24" @default.
• W1977711214 startingPage "S23" @default.
• W1977711214 abstract "ObjectiveHoxa-10-/- mice have severely compromised fertility from aberrant Dcz. CycD3, G1-phase cell cycle protein involved in stromal cell Dcz, is significantly reduced in Hoxa-10-/- mice. Our objective was to determine whether adenovirus-driven replacement of CycD3 can improve Dcz in Hoxa-10-/- mice both in vitro & in vivo.DesignExperimental study.Materials and methodsHoxa-10-/- stromal cell culture infected with empty (rAd-GFP) (control) or CycD3 sense (rAd-CycD3) vectors was assessed at 24 and 72h after Dcz conditioned medium treatment by immunostaining & proteomics. Hoxa-10-/- mice treated with rAd-GFP or rAd-CycD3 on d5 were sacrificed on d8 (n=6, 6), d10 (n=5, 5) and d12 [n=5 (rAd-CycD3 only)]. Implantation sites (IS) were assessed grossly, and with immunohistochemistry (IHC) & in situ hybridization (ISH).ResultsStromal cell culture infected with rAd-CycD3 had increased proliferation at 24 h & percentage of binucleation at 72 h than rAd-GFP (18.8%±1.7 vs. 6.2%±1.9, P<0.001). Proteomics demonstrated 14 up-regulated proteins; 6 at 24 h & 8 at 72 h in rAd-CycD3 culture vs. control. On d8 rAd-Cyc3 mice had larger IS weight vs. rAd-GFP (0.014g±0.004 vs. 0.003g±0 .002, P<0.001) & increased number of IS (5.7±2.7 vs. 1.4±1.0, P<0.001); larger weight was also seen at d10 (0.031±0.006 vs. 0.005±004, P<0.001). IHC demonstrated increased number of positively stained cells for proliferation & Dcz markers. ISH confirmed increased expression of Dcz markers. ISH at d10 found expression of placentation markers in rAd-CycD3 but not in rAd-GFP mice. At day 12, all IS of rAd-CycD3 mice had failed. ISH demonstrated that 7 genes identified via proteomics as down-stream targets of CycD3 had increased expression in the decidual bed at d7 compared with d4.ConclusionCycD3 overexpression improved Dcz in Hoxa-10-/- mice in both in vitro and in vivo models. However, we were unable to show reversal of pregnancy failure past d10 in Hoxa-10-/- mice. ObjectiveHoxa-10-/- mice have severely compromised fertility from aberrant Dcz. CycD3, G1-phase cell cycle protein involved in stromal cell Dcz, is significantly reduced in Hoxa-10-/- mice. Our objective was to determine whether adenovirus-driven replacement of CycD3 can improve Dcz in Hoxa-10-/- mice both in vitro & in vivo. Hoxa-10-/- mice have severely compromised fertility from aberrant Dcz. CycD3, G1-phase cell cycle protein involved in stromal cell Dcz, is significantly reduced in Hoxa-10-/- mice. Our objective was to determine whether adenovirus-driven replacement of CycD3 can improve Dcz in Hoxa-10-/- mice both in vitro & in vivo. DesignExperimental study. Experimental study. Materials and methodsHoxa-10-/- stromal cell culture infected with empty (rAd-GFP) (control) or CycD3 sense (rAd-CycD3) vectors was assessed at 24 and 72h after Dcz conditioned medium treatment by immunostaining & proteomics. Hoxa-10-/- mice treated with rAd-GFP or rAd-CycD3 on d5 were sacrificed on d8 (n=6, 6), d10 (n=5, 5) and d12 [n=5 (rAd-CycD3 only)]. Implantation sites (IS) were assessed grossly, and with immunohistochemistry (IHC) & in situ hybridization (ISH). Hoxa-10-/- stromal cell culture infected with empty (rAd-GFP) (control) or CycD3 sense (rAd-CycD3) vectors was assessed at 24 and 72h after Dcz conditioned medium treatment by immunostaining & proteomics. Hoxa-10-/- mice treated with rAd-GFP or rAd-CycD3 on d5 were sacrificed on d8 (n=6, 6), d10 (n=5, 5) and d12 [n=5 (rAd-CycD3 only)]. Implantation sites (IS) were assessed grossly, and with immunohistochemistry (IHC) & in situ hybridization (ISH). ResultsStromal cell culture infected with rAd-CycD3 had increased proliferation at 24 h & percentage of binucleation at 72 h than rAd-GFP (18.8%±1.7 vs. 6.2%±1.9, P<0.001). Proteomics demonstrated 14 up-regulated proteins; 6 at 24 h & 8 at 72 h in rAd-CycD3 culture vs. control. On d8 rAd-Cyc3 mice had larger IS weight vs. rAd-GFP (0.014g±0.004 vs. 0.003g±0 .002, P<0.001) & increased number of IS (5.7±2.7 vs. 1.4±1.0, P<0.001); larger weight was also seen at d10 (0.031±0.006 vs. 0.005±004, P<0.001). IHC demonstrated increased number of positively stained cells for proliferation & Dcz markers. ISH confirmed increased expression of Dcz markers. ISH at d10 found expression of placentation markers in rAd-CycD3 but not in rAd-GFP mice. At day 12, all IS of rAd-CycD3 mice had failed. ISH demonstrated that 7 genes identified via proteomics as down-stream targets of CycD3 had increased expression in the decidual bed at d7 compared with d4. Stromal cell culture infected with rAd-CycD3 had increased proliferation at 24 h & percentage of binucleation at 72 h than rAd-GFP (18.8%±1.7 vs. 6.2%±1.9, P<0.001). Proteomics demonstrated 14 up-regulated proteins; 6 at 24 h & 8 at 72 h in rAd-CycD3 culture vs. control. On d8 rAd-Cyc3 mice had larger IS weight vs. rAd-GFP (0.014g±0.004 vs. 0.003g±0 .002, P<0.001) & increased number of IS (5.7±2.7 vs. 1.4±1.0, P<0.001); larger weight was also seen at d10 (0.031±0.006 vs. 0.005±004, P<0.001). IHC demonstrated increased number of positively stained cells for proliferation & Dcz markers. ISH confirmed increased expression of Dcz markers. ISH at d10 found expression of placentation markers in rAd-CycD3 but not in rAd-GFP mice. At day 12, all IS of rAd-CycD3 mice had failed. ISH demonstrated that 7 genes identified via proteomics as down-stream targets of CycD3 had increased expression in the decidual bed at d7 compared with d4. ConclusionCycD3 overexpression improved Dcz in Hoxa-10-/- mice in both in vitro and in vivo models. However, we were unable to show reversal of pregnancy failure past d10 in Hoxa-10-/- mice. CycD3 overexpression improved Dcz in Hoxa-10-/- mice in both in vitro and in vivo models. However, we were unable to show reversal of pregnancy failure past d10 in Hoxa-10-/- mice." @default.
• W1977711214 created "2016-06-24" @default.
• W1977711214 creator A5009513853 @default.
• W1977711214 creator A5044765532 @default.
• W1977711214 creator A5075043640 @default.
• W1977711214 creator A5081454701 @default.
• W1977711214 date "2012-09-01" @default.
• W1977711214 modified "2023-10-16" @default.
• W1977711214 title "Replaceable cyclin D3 (CycD3) improves decidualization (Dcz) defects in HOXA-10-/- mice" @default.
• W1977711214 doi "https://doi.org/10.1016/j.fertnstert.2012.07.087" @default.
• W1977711214 hasPublicationYear "2012" @default.
• W1977711214 type Work @default.
• W1977711214 sameAs 1977711214 @default.
• W1977711214 citedByCount "0" @default.
• W1977711214 crossrefType "journal-article" @default.
• W1977711214 hasAuthorship W1977711214A5009513853 @default.
• W1977711214 hasAuthorship W1977711214A5044765532 @default.
• W1977711214 hasAuthorship W1977711214A5075043640 @default.
• W1977711214 hasAuthorship W1977711214A5081454701 @default.
• W1977711214 hasBestOaLocation W19777112141 @default.
• W1977711214 hasConcept C104317684 @default.
• W1977711214 hasConcept C1491633281 @default.
• W1977711214 hasConcept C150194340 @default.
• W1977711214 hasConcept C153911025 @default.
• W1977711214 hasConcept C16685009 @default.
• W1977711214 hasConcept C16930146 @default.
• W1977711214 hasConcept C185592680 @default.
• W1977711214 hasConcept C203014093 @default.
• W1977711214 hasConcept C204232928 @default.
• W1977711214 hasConcept C29537977 @default.
• W1977711214 hasConcept C4224716 @default.
• W1977711214 hasConcept C502942594 @default.
• W1977711214 hasConcept C55493867 @default.
• W1977711214 hasConcept C62112901 @default.
• W1977711214 hasConcept C71924100 @default.
• W1977711214 hasConcept C81439078 @default.
• W1977711214 hasConcept C86803240 @default.
• W1977711214 hasConceptScore W1977711214C104317684 @default.
• W1977711214 hasConceptScore W1977711214C1491633281 @default.
• W1977711214 hasConceptScore W1977711214C150194340 @default.
• W1977711214 hasConceptScore W1977711214C153911025 @default.
• W1977711214 hasConceptScore W1977711214C16685009 @default.
• W1977711214 hasConceptScore W1977711214C16930146 @default.
• W1977711214 hasConceptScore W1977711214C185592680 @default.
• W1977711214 hasConceptScore W1977711214C203014093 @default.
• W1977711214 hasConceptScore W1977711214C204232928 @default.
• W1977711214 hasConceptScore W1977711214C29537977 @default.
• W1977711214 hasConceptScore W1977711214C4224716 @default.
• W1977711214 hasConceptScore W1977711214C502942594 @default.
• W1977711214 hasConceptScore W1977711214C55493867 @default.
• W1977711214 hasConceptScore W1977711214C62112901 @default.
• W1977711214 hasConceptScore W1977711214C71924100 @default.
• W1977711214 hasConceptScore W1977711214C81439078 @default.
• W1977711214 hasConceptScore W1977711214C86803240 @default.
• W1977711214 hasFunder F4320332161 @default.
• W1977711214 hasIssue "3" @default.
• W1977711214 hasLocation W19777112141 @default.
• W1977711214 hasOpenAccess W1977711214 @default.
• W1977711214 hasPrimaryLocation W19777112141 @default.
• W1977711214 hasRelatedWork W2013912939 @default.
• W1977711214 hasRelatedWork W2026530198 @default.
• W1977711214 hasRelatedWork W2049824540 @default.
• W1977711214 hasRelatedWork W2054271113 @default.
• W1977711214 hasRelatedWork W207830445 @default.
• W1977711214 hasRelatedWork W2134995196 @default.
• W1977711214 hasRelatedWork W2353050464 @default.
• W1977711214 hasRelatedWork W2382915535 @default.
• W1977711214 hasRelatedWork W2401196811 @default.
• W1977711214 hasRelatedWork W60198975 @default.
• W1977711214 hasVolume "98" @default.
• W1977711214 isParatext "false" @default.
• W1977711214 isRetracted "false" @default.
• W1977711214 magId "1977711214" @default.
• W1977711214 workType "article" @default.