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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1977714180> ?p ?o ?g. }

• W1977714180 abstract "We present a method for the isolation of YAC insert sequences by representational difference analysis (RDA). To achieve maximal representation of the sequences, the amplicons were generated from a Mbol digestion product. RDA was performed using a 970 kb insert YAC clone. After two rounds of re-association and selective amplification 92% of the difference product represented sequences derived from the YAC insert. Twenty insert-specific sequence-tagged sites were readily defined. The difference product was also successfully used to isolate microsatellite markers, to identify clones from a human PAC library and as a chromosome painting probe in fluorescence in situ hybridization." @default.
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• W1977714180 date "1995-01-01" @default.
• W1977714180 modified "2023-09-27" @default.
• W1977714180 title "Isolation of YAC insert sequences by representational difference analysis" @default.
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• W1977714180 doi "https://doi.org/10.1093/nar/23.20.4127" @default.
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