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- W1977722804 abstract "Novikoff hepatocellular carcinoma cells possess cell-surface glycoproteins that bind the lectin, concanavalin A. A subset of Con A-binding plasma membrane glycoproteins was solubilized by addition of n -butanol to a suspension of Novikoff cells. Glycoproteins solubilized into the n -butanol-saturated aqueous phase of the two-phase mixture were purified by sequential chromatography on DEAE-cellulose and Sepharose-conjugated concanavalin A. Glycoproteins specifically bound to the Sepharose-conjugated Con A exhibited apparent M r = 72,000 to 125,000. The plasma membrane localization of these components was inferred by their isolation from cells surface labeled with NaIO 4 / NaB 3 H 4 . A xenoantiserum, raised against glycoproteins specifically bound to Sepharose-conjugated concanavalin A was employed to identify reactive components in nonionic detergent extracts of Novikoff tumor cells or rat hepatocytes surface labeled using lactoperoxidase-catalyzed iodination ( 125 I). Major reactive peptides in extracts of Novikoff cells exhibited apparent M r = 74,000, 82, 000, 110,000, and 135,000, while those in extracts of hepatocytes possessed apparent M r = 98,000 and 105,000. The reactivity of the antiserum with extracts of 125 I-labeled Novikoff cells was abolished by absorption of the antiserum with hepatocytes, indicating that the qualitative differences observed may result from structural modification of one or more cell-surface glycoproteins, rather than the expression of new or inappropriate glycoproteins. This antiserum will provide a useful probe to investigate alterations in the expression or structure of glycoproteins that occur as a consequence of malignant transformation or adaptation of malignant cells to growth in the ascitic form." @default.
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- W1977722804 date "1982-12-01" @default.
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- W1977722804 title "Selective solubilization and purification of cell-surface concanavalin A-binding glycoproteins from Novikoff hepatocellular carcinoma cells" @default.
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- W1977722804 doi "https://doi.org/10.1016/0003-9861(82)90173-4" @default.
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