FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1977797303> ?p ?o ?g. }

• W1977797303 endingPage "850" @default.
• W1977797303 startingPage "839" @default.
• W1977797303 abstract "We present a simple and safe strategy for producing high-titer adeno-associated virus (AAV) vectors derived from six different AAV serotypes (AAV-1 to AAV-6). The method, referred to as HOT, is helper virus free, optically controllable, and based on transfection of only two plasmids, i.e., an AAV vector construct and one of six novel AAV helper plasmids. The latter were engineered to carry AAV serotype rep and cap genes together with adenoviral helper functions, as well as unique fluorescent protein expression cassettes, allowing confirmation of successful transfection and identification of the transfected plasmid. Cross-packaging of vector DNA derived from AAV-2, -3, or -6 was up to 10-fold more efficient using our novel plasmids, compared to a conservative adenovirus-dependent method. We also identified a variety of useful antibodies, allowing detection of Rep or VP proteins, or assembled capsids, of all six AAV serotypes. Finally, we describe unique cell tropisms and kinetics of transgene expression for AAV serotype vectors in primary or transformed cells from four different species. In sum, the HOT strategy and the antibodies presented here, together with the reported findings, should facilitate and support the further development of AAV serotype vectors as powerful new tools for human gene therapy." @default.
• W1977797303 created "2016-06-24" @default.
• W1977797303 creator A5023767037 @default.
• W1977797303 creator A5027557554 @default.
• W1977797303 creator A5055110680 @default.
• W1977797303 date "2003-06-01" @default.
• W1977797303 modified "2023-10-14" @default.
• W1977797303 title "Helper virus-free, optically controllable, and two-plasmid-based production of adeno-associated virus vectors of serotypes 1 to 6" @default.
• W1977797303 cites W1490724066 @default.
• W1977797303 cites W1895050046 @default.
• W1977797303 cites W1922255142 @default.
• W1977797303 cites W1963623233 @default.
• W1977797303 cites W1975528602 @default.
• W1977797303 cites W1995691954 @default.
• W1977797303 cites W2004983645 @default.
• W1977797303 cites W2044005085 @default.
• W1977797303 cites W2047608515 @default.
• W1977797303 cites W2050245606 @default.
• W1977797303 cites W2051072233 @default.
• W1977797303 cites W2051159468 @default.
• W1977797303 cites W2053706844 @default.
• W1977797303 cites W2059108053 @default.
• W1977797303 cites W2079090019 @default.
• W1977797303 cites W2090661761 @default.
• W1977797303 cites W2096309023 @default.
• W1977797303 cites W2106803982 @default.
• W1977797303 cites W2115185064 @default.
• W1977797303 cites W2118531658 @default.
• W1977797303 cites W2119056879 @default.
• W1977797303 cites W2120193263 @default.
• W1977797303 cites W2134766536 @default.
• W1977797303 cites W2150650564 @default.
• W1977797303 cites W2151514148 @default.
• W1977797303 cites W2154329712 @default.
• W1977797303 cites W2154330857 @default.
• W1977797303 cites W2158647587 @default.
• W1977797303 cites W2168823331 @default.
• W1977797303 cites W2172045470 @default.
• W1977797303 cites W4233745600 @default.
• W1977797303 cites W4245307762 @default.
• W1977797303 doi "https://doi.org/10.1016/s1525-0016(03)00095-9" @default.
• W1977797303 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12788658" @default.
• W1977797303 hasPublicationYear "2003" @default.
• W1977797303 type Work @default.
• W1977797303 sameAs 1977797303 @default.
• W1977797303 citedByCount "310" @default.
• W1977797303 countsByYear W19777973032012 @default.
• W1977797303 countsByYear W19777973032013 @default.
• W1977797303 countsByYear W19777973032014 @default.
• W1977797303 countsByYear W19777973032015 @default.
• W1977797303 countsByYear W19777973032016 @default.
• W1977797303 countsByYear W19777973032017 @default.
• W1977797303 countsByYear W19777973032018 @default.
• W1977797303 countsByYear W19777973032019 @default.
• W1977797303 countsByYear W19777973032020 @default.
• W1977797303 countsByYear W19777973032021 @default.
• W1977797303 countsByYear W19777973032022 @default.
• W1977797303 countsByYear W19777973032023 @default.
• W1977797303 crossrefType "journal-article" @default.
• W1977797303 hasAuthorship W1977797303A5023767037 @default.
• W1977797303 hasAuthorship W1977797303A5027557554 @default.
• W1977797303 hasAuthorship W1977797303A5055110680 @default.
• W1977797303 hasBestOaLocation W19777973031 @default.
• W1977797303 hasConcept C102230213 @default.
• W1977797303 hasConcept C10389963 @default.
• W1977797303 hasConcept C104317684 @default.
• W1977797303 hasConcept C111599444 @default.
• W1977797303 hasConcept C135983454 @default.
• W1977797303 hasConcept C140704245 @default.
• W1977797303 hasConcept C144001008 @default.
• W1977797303 hasConcept C15152581 @default.
• W1977797303 hasConcept C153911025 @default.
• W1977797303 hasConcept C159047783 @default.
• W1977797303 hasConcept C202878990 @default.
• W1977797303 hasConcept C22744801 @default.
• W1977797303 hasConcept C2522874641 @default.
• W1977797303 hasConcept C2778107364 @default.
• W1977797303 hasConcept C32611913 @default.
• W1977797303 hasConcept C40767141 @default.
• W1977797303 hasConcept C54009773 @default.
• W1977797303 hasConcept C54355233 @default.
• W1977797303 hasConcept C55493867 @default.
• W1977797303 hasConcept C86803240 @default.
• W1977797303 hasConcept C92087593 @default.
• W1977797303 hasConceptScore W1977797303C102230213 @default.
• W1977797303 hasConceptScore W1977797303C10389963 @default.
• W1977797303 hasConceptScore W1977797303C104317684 @default.
• W1977797303 hasConceptScore W1977797303C111599444 @default.
• W1977797303 hasConceptScore W1977797303C135983454 @default.
• W1977797303 hasConceptScore W1977797303C140704245 @default.
• W1977797303 hasConceptScore W1977797303C144001008 @default.
• W1977797303 hasConceptScore W1977797303C15152581 @default.
• W1977797303 hasConceptScore W1977797303C153911025 @default.
• W1977797303 hasConceptScore W1977797303C159047783 @default.
• W1977797303 hasConceptScore W1977797303C202878990 @default.
• W1977797303 hasConceptScore W1977797303C22744801 @default.
• W1977797303 hasConceptScore W1977797303C2522874641 @default.
• W1977797303 hasConceptScore W1977797303C2778107364 @default.

• next