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- W1977813518 abstract "In experiments using preparations containing 1, 2, or 3 twitch fibers dissected from the semitendinosus muscle, the twitch was eliminated or greatly reduced (i.e., <10% of control) when the preparations were exposed for < 10 min to a OCa++, bicarbonate-buffered Ringer's solution (37 out of 41 experiments). Replacing the bicarbonate buffer by a tris buffer delayed the loss of the twitch in OCa++ solutions. An even greater delay was produced by substituting a phosphate buffer for the bicarbonate. In 7 out of 12 experiments, twitches of control size or larger continued for 8 to 18 min in a OCa++, phosphate-buffered solution; in the other 5 experiments the twitch was reduced or eliminated < 8 min. The loss of the twitch takes much longer than required for removal of extracellular Ca++ ions from the t-tubules and much shorter than required for reduction of the sarcoplasmic reticular stores of Ca++. The results support the ‘trigger Ca++’ hypothesis which proposes that t-tubular membrane-bound Ca++ ions play an essential role in excitation-contraction coupling." @default.
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- W1977813518 date "1978-03-01" @default.
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- W1977813518 title "Rapid loss of the twitch of frog's skeletal muscle fibers in OCa++ bicarbonate-buffered ringer's solution" @default.
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- W1977813518 doi "https://doi.org/10.1016/0024-3205(78)90277-1" @default.
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