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• W1977843417 abstract "Selective activation of mitoKATP channels can protect the brain or cultured neurons against a variety of anoxic or metabolic challenges. However, little is known about the subunit composition or functional regulation of the channel itself. In the present study, we sought to characterize the mitoKATP channel in the mouse brain using overlapping approaches. First, we determined that mitochondria contain the pore-forming Kir6.1 and Kir6.2 subunits with Western blotting, immunogold electron microscopy and the identification of mitochondrial transport sequences. In contrast, we found no evidence for the presence of either known sulfonylurea receptors (SUR1 or SUR2) in the mitochondria. However, the ATP-dependent K (KATP) channel inhibitor glibenclamide specifically binds to mitochondria in both neurons and astrocytes, and small molecular weight SUR2-like proteins were concentrated in mitochondria. In addition to mice, similar results were found in rats and pigs. Second, live respiring mitochondria were stained with the membrane potential sensitive dye MitoFluorRed and visualized by confocal microscopy. We investigated the effects of pharmacological closing and opening of the channel with glibenclamide and the specific mitoKATP openers diazoxide and BMS-191095. Closing of the channel inhibited the energization of the mitochondria, which was reversed by the application of the mitoKATP openers. We also found that blocking mitochondrial peroxynitrite formation with FP15 has a similar effect to blocking the mitoKATP channels. We conclude that brain mitochondria contain functional KATP channels. The pore-forming subunit of the channel can be either Kir6.1 or Kir6.2, and the SUR subunit may be a SUR2 splice variant or a similar protein." @default.
• W1977843417 created "2016-06-24" @default.
• W1977843417 creator A5010556646 @default.
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• W1977843417 date "2003-12-01" @default.
• W1977843417 modified "2023-10-17" @default.
• W1977843417 title "Investigation of the subunit composition and the pharmacology of the mitochondrial ATP-dependent K+ channel in the brain" @default.
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• W1977843417 doi "https://doi.org/10.1016/j.brainres.2003.09.046" @default.
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