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• W1977919602 abstract "The FASTPlaqueTB assay is an established diagnostic aid for the rapid detection of Mycobacterium tuberculosis from human sputum samples. Using the FASTPlaqueTB assay reagents, viable Mycobacterium avium subsp. paratuberculosis cells were detected as phage plaques in just 24 h. The bacteriophage used does not infect M. avium subsp. paratuberculosis alone, so to add specificity to this assay, a PCR-based identification method was introduced to amplify M. avium subsp. paratuberculosis-specific sequences from the DNA of the mycobacterial cell detected by the phage. To give further diagnostic information, a multiplex PCR method was developed to allow simultaneous amplification of either M. avium subsp. paratuberculosis or M. tuberculosis complex-specific sequences from plaque samples. Combining the plaque PCR technique with the phage-based detection assay allowed the rapid and specific detection of viable M. avium subsp. paratuberculosis in milk samples in just 48 h." @default.
• W1977919602 created "2016-06-24" @default.
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• W1977919602 date "2007-03-15" @default.
• W1977919602 modified "2023-10-12" @default.
• W1977919602 title "Development of a New, Combined Rapid Method Using Phage and PCR for Detection and Identification of Viable <i>Mycobacterium paratuberculosis</i> Bacteria within 48 Hours" @default.
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• W1977919602 doi "https://doi.org/10.1128/aem.01722-06" @default.
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