FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1977990208> ?p ?o ?g. }

• W1977990208 endingPage "318" @default.
• W1977990208 startingPage "312" @default.
• W1977990208 abstract "Alcohol-induced activation of penicillin V acylase from Streptomyces lavendulae by some water-miscible primary alcohols such as methanol, ethanol, 1-propanol and 1-butanol has been extensively studied. The effects of various concentrations of these alcohols on the maximum velocity of penicillin V hydrolysis (Vmax) and the apparent Michaelis-Menten constant (Km) of the enzyme are reported. Lineweaver-Burk analyses of the kinetic data revealed a phenomenon of nonessential activation in the presence of short-chain primary alcohols. In the concentration range where activation was observed, conventional secondary replots of 1/Δ slope or 1/Δ intercept versus 1/[alcohol] yielded perfect straight lines, demonstrating the binding of a single molecule of alcohol to an activator site in penicillin V acylase. Furthermore, the enzyme displayed a propensity to bind the longer chain alcohols, as reflected by the dissociation constant KA values of 493, 369, 228 and 11.8 mM for methanol, ethanol, 1-propanol and 1-butanol, respectively. Therefore, we have proposed a high affinity activator site for alcohols in the enzyme. Very hydrophobic alcohols such as 1-butanol bind more efficiently to this activator site and convert the enzyme form one conformation to another one, which is catalytically more effective than the alcohol-free form of the penicillin acylase. Further addition of alcohol results in a competitive inhibition of the enzyme in its activated state. An inverse relationship between the chain length of the alcohols and the breakpoints in the biphasic normalized vmax/Km ratio versus alcohol concentration plots was observed." @default.
• W1977990208 created "2016-06-24" @default.
• W1977990208 creator A5004282738 @default.
• W1977990208 creator A5017381614 @default.
• W1977990208 creator A5041963900 @default.
• W1977990208 creator A5053724886 @default.
• W1977990208 creator A5065357281 @default.
• W1977990208 creator A5086428854 @default.
• W1977990208 date "2001-09-01" @default.
• W1977990208 modified "2023-10-02" @default.
• W1977990208 title "Kinetic mechanism of penicillin V acylase activation byshort-chain alcohols" @default.
• W1977990208 cites W1590446381 @default.
• W1977990208 cites W1636206934 @default.
• W1977990208 cites W1916997903 @default.
• W1977990208 cites W1977857502 @default.
• W1977990208 cites W1978637852 @default.
• W1977990208 cites W1979422740 @default.
• W1977990208 cites W1981119403 @default.
• W1977990208 cites W1993583696 @default.
• W1977990208 cites W1994071890 @default.
• W1977990208 cites W1999770416 @default.
• W1977990208 cites W2007037633 @default.
• W1977990208 cites W2008871903 @default.
• W1977990208 cites W2026606414 @default.
• W1977990208 cites W2038346185 @default.
• W1977990208 cites W2044430464 @default.
• W1977990208 cites W2053362950 @default.
• W1977990208 cites W2069462480 @default.
• W1977990208 cites W2089520347 @default.
• W1977990208 cites W2097408513 @default.
• W1977990208 cites W2114828970 @default.
• W1977990208 cites W2152427814 @default.
• W1977990208 cites W4293247451 @default.
• W1977990208 cites W44410246 @default.
• W1977990208 doi "https://doi.org/10.1016/s0141-0229(01)00391-x" @default.
• W1977990208 hasPublicationYear "2001" @default.
• W1977990208 type Work @default.
• W1977990208 sameAs 1977990208 @default.
• W1977990208 citedByCount "2" @default.
• W1977990208 crossrefType "journal-article" @default.
• W1977990208 hasAuthorship W1977990208A5004282738 @default.
• W1977990208 hasAuthorship W1977990208A5017381614 @default.
• W1977990208 hasAuthorship W1977990208A5041963900 @default.
• W1977990208 hasAuthorship W1977990208A5053724886 @default.
• W1977990208 hasAuthorship W1977990208A5065357281 @default.
• W1977990208 hasAuthorship W1977990208A5086428854 @default.
• W1977990208 hasConcept C121332964 @default.
• W1977990208 hasConcept C148898269 @default.
• W1977990208 hasConcept C170493617 @default.
• W1977990208 hasConcept C174802600 @default.
• W1977990208 hasConcept C178790620 @default.
• W1977990208 hasConcept C181199279 @default.
• W1977990208 hasConcept C185592680 @default.
• W1977990208 hasConcept C2776452521 @default.
• W1977990208 hasConcept C2777451570 @default.
• W1977990208 hasConcept C2777713963 @default.
• W1977990208 hasConcept C2779607525 @default.
• W1977990208 hasConcept C2780161600 @default.
• W1977990208 hasConcept C2781066024 @default.
• W1977990208 hasConcept C28212737 @default.
• W1977990208 hasConcept C33633552 @default.
• W1977990208 hasConcept C501593827 @default.
• W1977990208 hasConcept C55493867 @default.
• W1977990208 hasConcept C62520636 @default.
• W1977990208 hasConcept C64915919 @default.
• W1977990208 hasConcept C71240020 @default.
• W1977990208 hasConcept C74998103 @default.
• W1977990208 hasConcept C93391505 @default.
• W1977990208 hasConcept C94412978 @default.
• W1977990208 hasConceptScore W1977990208C121332964 @default.
• W1977990208 hasConceptScore W1977990208C148898269 @default.
• W1977990208 hasConceptScore W1977990208C170493617 @default.
• W1977990208 hasConceptScore W1977990208C174802600 @default.
• W1977990208 hasConceptScore W1977990208C178790620 @default.
• W1977990208 hasConceptScore W1977990208C181199279 @default.
• W1977990208 hasConceptScore W1977990208C185592680 @default.
• W1977990208 hasConceptScore W1977990208C2776452521 @default.
• W1977990208 hasConceptScore W1977990208C2777451570 @default.
• W1977990208 hasConceptScore W1977990208C2777713963 @default.
• W1977990208 hasConceptScore W1977990208C2779607525 @default.
• W1977990208 hasConceptScore W1977990208C2780161600 @default.
• W1977990208 hasConceptScore W1977990208C2781066024 @default.
• W1977990208 hasConceptScore W1977990208C28212737 @default.
• W1977990208 hasConceptScore W1977990208C33633552 @default.
• W1977990208 hasConceptScore W1977990208C501593827 @default.
• W1977990208 hasConceptScore W1977990208C55493867 @default.
• W1977990208 hasConceptScore W1977990208C62520636 @default.
• W1977990208 hasConceptScore W1977990208C64915919 @default.
• W1977990208 hasConceptScore W1977990208C71240020 @default.
• W1977990208 hasConceptScore W1977990208C74998103 @default.
• W1977990208 hasConceptScore W1977990208C93391505 @default.
• W1977990208 hasConceptScore W1977990208C94412978 @default.
• W1977990208 hasIssue "4-5" @default.
• W1977990208 hasLocation W19779902081 @default.
• W1977990208 hasOpenAccess W1977990208 @default.
• W1977990208 hasPrimaryLocation W19779902081 @default.
• W1977990208 hasRelatedWork W1973797179 @default.
• W1977990208 hasRelatedWork W1997762032 @default.

• next