FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1977996490> ?p ?o ?g. }

• W1977996490 endingPage "175" @default.
• W1977996490 startingPage "163" @default.
• W1977996490 abstract "Cells transfected with specific N-methyl-d-aspartate (NMDA) receptor subtypes undergo cell death that mimics glutamate-induced excitotoxicity pharmacologically. We have further characterized the mechanisms of cell death resulting from NMDA receptor activation in such cells through development of cell counting methods based on co-transfection with green fluorescent protein. When co-transfected with NMDA receptors, GFP expression was limited to live cells as indicated by the observation that GFP was only detected in cells which were positive for markers of live cells, and was found in no cells which were trypan blue or propidium iodide positive. Using co-transfection with green fluorescent protein and cell counting of viable cells with a fluorescence activated cells sorter, we confirmed the subunit-specific profile of NMDA receptor-mediated cell death in cells transfected with NMDA receptors. Toxicity was greatest in the NR1A/2A receptor, less in the NR1A/2B receptor, and least in NR1A/2C receptors. Cell death also differed pharmacologically between subunit combinations. Cell death in cells transfected with NR 1A/2A was blocked by amino-phosphonovaleric acid at lower concentrations than in cells transfected with NR 1A/2B. In cells transfected with the NR1A/2A or NR1A/2B combinations but not NR1A/2C, cell death was also associated with production of reactive oxygen species. In addition, removal of the final 400 amino acids of the C-terminal region of NR2A decreased cell death. The use of GFP based cell counting provides a sensitive mechanism for assessing the mechanism of excitotoxicity in transfected cell models." @default.
• W1977996490 created "2016-06-24" @default.
• W1977996490 creator A5010542220 @default.
• W1977996490 creator A5023685958 @default.
• W1977996490 creator A5038108146 @default.
• W1977996490 creator A5054748238 @default.
• W1977996490 creator A5078737409 @default.
• W1977996490 creator A5084824937 @default.
• W1977996490 date "2000-05-01" @default.
• W1977996490 modified "2023-10-16" @default.
• W1977996490 title "N-Methyl-d-aspartate receptor mediated toxicity in nonneuronal cell lines: characterization using fluorescent measures of cell viability and reactive oxygen species production" @default.
• W1977996490 cites W1556086693 @default.
• W1977996490 cites W1594111906 @default.
• W1977996490 cites W1608934724 @default.
• W1977996490 cites W1639407471 @default.
• W1977996490 cites W1653383134 @default.
• W1977996490 cites W1909841186 @default.
• W1977996490 cites W1954929088 @default.
• W1977996490 cites W1966673980 @default.
• W1977996490 cites W1975944686 @default.
• W1977996490 cites W1978137213 @default.
• W1977996490 cites W1983566389 @default.
• W1977996490 cites W1984049384 @default.
• W1977996490 cites W1990625157 @default.
• W1977996490 cites W2014091183 @default.
• W1977996490 cites W2017329375 @default.
• W1977996490 cites W2020569812 @default.
• W1977996490 cites W2026759345 @default.
• W1977996490 cites W2032008843 @default.
• W1977996490 cites W2053846841 @default.
• W1977996490 cites W2053980736 @default.
• W1977996490 cites W2059334396 @default.
• W1977996490 cites W2064477978 @default.
• W1977996490 cites W2065169750 @default.
• W1977996490 cites W2068196125 @default.
• W1977996490 cites W2071218335 @default.
• W1977996490 cites W2075450053 @default.
• W1977996490 cites W2077706363 @default.
• W1977996490 cites W2080213202 @default.
• W1977996490 cites W2085402579 @default.
• W1977996490 cites W2141550849 @default.
• W1977996490 cites W2152541148 @default.
• W1977996490 cites W2317018376 @default.
• W1977996490 doi "https://doi.org/10.1016/s0169-328x(00)00050-4" @default.
• W1977996490 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10837912" @default.
• W1977996490 hasPublicationYear "2000" @default.
• W1977996490 type Work @default.
• W1977996490 sameAs 1977996490 @default.
• W1977996490 citedByCount "37" @default.
• W1977996490 countsByYear W19779964902012 @default.
• W1977996490 countsByYear W19779964902013 @default.
• W1977996490 countsByYear W19779964902016 @default.
• W1977996490 countsByYear W19779964902017 @default.
• W1977996490 crossrefType "journal-article" @default.
• W1977996490 hasAuthorship W1977996490A5010542220 @default.
• W1977996490 hasAuthorship W1977996490A5023685958 @default.
• W1977996490 hasAuthorship W1977996490A5038108146 @default.
• W1977996490 hasAuthorship W1977996490A5054748238 @default.
• W1977996490 hasAuthorship W1977996490A5078737409 @default.
• W1977996490 hasAuthorship W1977996490A5084824937 @default.
• W1977996490 hasConcept C104317684 @default.
• W1977996490 hasConcept C142613039 @default.
• W1977996490 hasConcept C1491633281 @default.
• W1977996490 hasConcept C153911025 @default.
• W1977996490 hasConcept C170493617 @default.
• W1977996490 hasConcept C190283241 @default.
• W1977996490 hasConcept C2775934118 @default.
• W1977996490 hasConcept C2781012912 @default.
• W1977996490 hasConcept C31573885 @default.
• W1977996490 hasConcept C53227056 @default.
• W1977996490 hasConcept C54009773 @default.
• W1977996490 hasConcept C54355233 @default.
• W1977996490 hasConcept C55493867 @default.
• W1977996490 hasConcept C67018056 @default.
• W1977996490 hasConcept C81885089 @default.
• W1977996490 hasConcept C86803240 @default.
• W1977996490 hasConcept C95444343 @default.
• W1977996490 hasConceptScore W1977996490C104317684 @default.
• W1977996490 hasConceptScore W1977996490C142613039 @default.
• W1977996490 hasConceptScore W1977996490C1491633281 @default.
• W1977996490 hasConceptScore W1977996490C153911025 @default.
• W1977996490 hasConceptScore W1977996490C170493617 @default.
• W1977996490 hasConceptScore W1977996490C190283241 @default.
• W1977996490 hasConceptScore W1977996490C2775934118 @default.
• W1977996490 hasConceptScore W1977996490C2781012912 @default.
• W1977996490 hasConceptScore W1977996490C31573885 @default.
• W1977996490 hasConceptScore W1977996490C53227056 @default.
• W1977996490 hasConceptScore W1977996490C54009773 @default.
• W1977996490 hasConceptScore W1977996490C54355233 @default.
• W1977996490 hasConceptScore W1977996490C55493867 @default.
• W1977996490 hasConceptScore W1977996490C67018056 @default.
• W1977996490 hasConceptScore W1977996490C81885089 @default.
• W1977996490 hasConceptScore W1977996490C86803240 @default.
• W1977996490 hasConceptScore W1977996490C95444343 @default.
• W1977996490 hasIssue "2" @default.
• W1977996490 hasLocation W19779964901 @default.
• W1977996490 hasLocation W19779964902 @default.
• W1977996490 hasOpenAccess W1977996490 @default.

• next