FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978025097> ?p ?o ?g. }

• W1978025097 endingPage "3540" @default.
• W1978025097 startingPage "3530" @default.
• W1978025097 abstract "Abstract Objective To investigate the effects of prostaglandin D 2 (PGD 2 ) on interleukin‐1β (IL‐1β)–induced matrix metalloproteinase 1 (MMP‐1) and MMP‐13 expression in human chondrocytes and the signaling pathways involved in these effects. Methods Chondrocytes were stimulated with IL‐1 in the presence or absence of PGD 2 , and expression of MMP‐1 and MMP‐13 proteins was evaluated by enzyme‐linked immunosorbent assay. Messenger RNA (mRNA) expression and promoter activity were analyzed by real‐time reverse transcription–polymerase chain reaction and transient transfections, respectively. The role of the PGD 2 receptors D prostanoid receptor 1 (DP1) and chemoattractant receptor–like molecule expressed on Th2 cells (CRTH2) was evaluated using specific agonists and antibody‐blocking experiments. The contribution of the cAMP/protein kinase A (PKA) pathway was determined using cAMP‐elevating agents and PKA inhibitors. Results PGD 2 decreased in a dose‐dependent manner IL‐1–induced MMP‐1 and MMP‐13 protein and mRNA expression as well as their promoter activation. DP1 and CRTH2 were expressed and functional in chondrocytes. The effect of PGD 2 was mimicked by BW245C, a selective agonist of DP1, but not by 13,14‐dihydro‐15‐keto‐PGD 2 , a selective agonist of CRTH2. Furthermore, treatment with an anti‐DP1 antibody reversed the effect of PGD 2 , indicating that the inhibitory effect of PGD 2 is mediated by DP1. The cAMP‐elevating agents 8‐Br‐cAMP and forskolin suppressed IL‐1–induced MMP‐1 and MMP‐13 expression, and the PKA inhibitors KT5720 and H89 reversed the inhibitory effect of PGD 2 , suggesting that the effect of PGD 2 is mediated by the cAMP/PKA pathway. Conclusion PGD 2 inhibits IL‐1–induced production of MMP‐1 and MMP‐13 by chondrocytes through the DP1/cAMP/PKA signaling pathway. These data also suggest that modulation of PGD 2 levels in the joint may have therapeutic potential in the prevention of cartilage degradation." @default.
• W1978025097 created "2016-06-24" @default.
• W1978025097 creator A5026711692 @default.
• W1978025097 creator A5032321171 @default.
• W1978025097 creator A5033883809 @default.
• W1978025097 creator A5039776422 @default.
• W1978025097 creator A5051212787 @default.
• W1978025097 creator A5052591744 @default.
• W1978025097 creator A5053214598 @default.
• W1978025097 creator A5061372270 @default.
• W1978025097 creator A5083125761 @default.
• W1978025097 creator A5088257611 @default.
• W1978025097 creator A5091353622 @default.
• W1978025097 date "2008-10-30" @default.
• W1978025097 modified "2023-10-16" @default.
• W1978025097 title "Inhibition of interleukin-1β-induced matrix metalloproteinases 1 and 13 production in human osteoarthritic chondrocytes by prostaglandin D₂" @default.
• W1978025097 cites W1488444587 @default.
• W1978025097 cites W1539392991 @default.
• W1978025097 cites W1555610421 @default.
• W1978025097 cites W1581863768 @default.
• W1978025097 cites W1586814698 @default.
• W1978025097 cites W1964073342 @default.
• W1978025097 cites W1966728300 @default.
• W1978025097 cites W1969179229 @default.
• W1978025097 cites W1971255663 @default.
• W1978025097 cites W1976234470 @default.
• W1978025097 cites W1976808483 @default.
• W1978025097 cites W1979972616 @default.
• W1978025097 cites W1980411055 @default.
• W1978025097 cites W1984483611 @default.
• W1978025097 cites W1992724479 @default.
• W1978025097 cites W1993181710 @default.
• W1978025097 cites W2001127228 @default.
• W1978025097 cites W2014972722 @default.
• W1978025097 cites W2026915010 @default.
• W1978025097 cites W2042738623 @default.
• W1978025097 cites W2043250054 @default.
• W1978025097 cites W2046453134 @default.
• W1978025097 cites W2046932891 @default.
• W1978025097 cites W2050906978 @default.
• W1978025097 cites W2051259340 @default.
• W1978025097 cites W2058147305 @default.
• W1978025097 cites W2063347675 @default.
• W1978025097 cites W2065860456 @default.
• W1978025097 cites W2077555941 @default.
• W1978025097 cites W2079644509 @default.
• W1978025097 cites W2087090665 @default.
• W1978025097 cites W2088146922 @default.
• W1978025097 cites W2092392094 @default.
• W1978025097 cites W2095222179 @default.
• W1978025097 cites W2103017931 @default.
• W1978025097 cites W2105825531 @default.
• W1978025097 cites W2112368404 @default.
• W1978025097 cites W2134407449 @default.
• W1978025097 cites W2140978174 @default.
• W1978025097 cites W2166587026 @default.
• W1978025097 cites W2166893697 @default.
• W1978025097 cites W2441253044 @default.
• W1978025097 cites W2986903554 @default.
• W1978025097 cites W66301014 @default.
• W1978025097 doi "https://doi.org/10.1002/art.23958" @default.
• W1978025097 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2657141" @default.
• W1978025097 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18975308" @default.
• W1978025097 hasPublicationYear "2008" @default.
• W1978025097 type Work @default.
• W1978025097 sameAs 1978025097 @default.
• W1978025097 citedByCount "53" @default.
• W1978025097 countsByYear W19780250972012 @default.
• W1978025097 countsByYear W19780250972013 @default.
• W1978025097 countsByYear W19780250972014 @default.
• W1978025097 countsByYear W19780250972015 @default.
• W1978025097 countsByYear W19780250972016 @default.
• W1978025097 countsByYear W19780250972017 @default.
• W1978025097 countsByYear W19780250972018 @default.
• W1978025097 countsByYear W19780250972019 @default.
• W1978025097 countsByYear W19780250972020 @default.
• W1978025097 countsByYear W19780250972021 @default.
• W1978025097 countsByYear W19780250972022 @default.
• W1978025097 countsByYear W19780250972023 @default.
• W1978025097 crossrefType "journal-article" @default.
• W1978025097 hasAuthorship W1978025097A5026711692 @default.
• W1978025097 hasAuthorship W1978025097A5032321171 @default.
• W1978025097 hasAuthorship W1978025097A5033883809 @default.
• W1978025097 hasAuthorship W1978025097A5039776422 @default.
• W1978025097 hasAuthorship W1978025097A5051212787 @default.
• W1978025097 hasAuthorship W1978025097A5052591744 @default.
• W1978025097 hasAuthorship W1978025097A5053214598 @default.
• W1978025097 hasAuthorship W1978025097A5061372270 @default.
• W1978025097 hasAuthorship W1978025097A5083125761 @default.
• W1978025097 hasAuthorship W1978025097A5088257611 @default.
• W1978025097 hasAuthorship W1978025097A5091353622 @default.
• W1978025097 hasBestOaLocation W19780250972 @default.
• W1978025097 hasConcept C104317684 @default.
• W1978025097 hasConcept C105580179 @default.
• W1978025097 hasConcept C109523444 @default.
• W1978025097 hasConcept C134018914 @default.
• W1978025097 hasConcept C153911025 @default.
• W1978025097 hasConcept C170493617 @default.

• next