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- W1978028272 abstract "We amplified the coding region DNA sequence from a rat renal pI(Cln) cDNA by PCR and expressed the protein in Escherichia coli cells. The cells were exposed to hypotonic conditions followed by spreading them onto LB plates for subsequent colony survival assay. The present study demonstrated that the cells expressing pI(Cln) exhibit a strong resistance to hypotonic stress. Moreover, the resistance was specifically inhibited by extracellular ATP and some anion channel inhibitors. These findings indicate that the expression of pI(Cln) directly confers tolerance to hypotonic stress, and pI(Cln) is concluded to be an important molecule for cell-volume regulation." @default.
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- W1978028272 date "1998-08-28" @default.
- W1978028272 modified "2023-10-16" @default.
- W1978028272 title "Expression of pIClninEscherichia coligives a strong tolerance to hypotonic stress" @default.
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- W1978028272 doi "https://doi.org/10.1016/s0014-5793(98)00946-6" @default.
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