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• W1978030932 abstract "Chromatographic zone broadening is a common issue in microfluidic chromatography, where the sample volume introduced on column often exceeds the column void volume. To better understand the propagation of wide chromatographic zones on a separation device, a series of MS Excel spreadsheets were developed to simulate the process. To computationally simplify these simulations, we investigated the effects of injection related zone broadening and its gradient related zone compression by tracking only the movements of zone boundaries on column. The effects of sample volume, sample solvent, gradient slope, and column length on zone broadening were evaluated and compared to experiments performed on 0.32 mm I.D. microfluidic columns. The repetitive injection method (RIM) was implemented to generate experimental chromatograms where large sample volume scenarios can be emulated by injecting two discrete small injection plugs spaced in time. A good match between predicted and experimental RIM chromatograms was observed. We discuss the performance of selected retention models on the accuracy of predictions and use the developed spreadsheets for illustration of gradient zone focusing for both small molecules and peptides." @default.
• W1978030932 created "2016-06-24" @default.
• W1978030932 creator A5014656792 @default.
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• W1978030932 date "2015-04-01" @default.
• W1978030932 modified "2023-10-18" @default.
• W1978030932 title "Wide injection zone compression in gradient reversed-phase liquid chromatography" @default.
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• W1978030932 doi "https://doi.org/10.1016/j.chroma.2015.02.057" @default.
• W1978030932 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25748538" @default.
• W1978030932 hasPublicationYear "2015" @default.
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