Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978083919> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W1978083919 endingPage "13377" @default.
- W1978083919 startingPage "13368" @default.
- W1978083919 abstract "Control of the balance between receptor activation and inactivation is a prerequisite for seven transmembrane domain (7TM) receptor function. We asked for a mechanism to stabilize the inactive receptor conformation which prevents agonist-independent receptor activation. Na+ ions have reciprocal effects on agonist versus antagonist interaction with various 7TM receptors. To investigate the Na+ dependence of receptor activation we chose the bradykinin B2 receptor as a prototypic 7TM receptor. Decrease of the intracellular Na+ content from 40 mM to 10 mM of COS-1 cells transiently expressing rat B2 receptors activated the B2 receptor in the absence of agonist as shown by a 3-fold increase in the basal release of inositolphosphates and increased the intrinsic activity of bradykinin to 1.2. In contrast, under increased intracellular Na+ (148 mM) the intrinsic activity of bradykinin decreased to 0.72. When the interaction of Na+ with the B2 receptor was prevented by exchanging a conserved aspartate in transmembrane domain II for asparagine the B2 receptor was also constitutively-activated in the absence of agonist. Agonist-independent B2 receptor activation under decreased intracellular Na+ was similarly observed with primary human fibroblasts endogenously expressing human B2 receptors by a 2.5-fold increase in basal inositolphosphates. Activation of human B2 receptors in the absence of agonist under decreased intracellular Na+ was further evident by an increased basal phosphorylation of the B2 receptor protein. Thus our data suggest that the interaction of Na+ ions with the B2 receptor stabilizes or induces an inactive receptor conformation thereby providing a mechanism to suppress agonist-independent receptor activation in vivo." @default.
- W1978083919 created "2016-06-24" @default.
- W1978083919 creator A5035742650 @default.
- W1978083919 creator A5052474412 @default.
- W1978083919 creator A5055302959 @default.
- W1978083919 creator A5075814041 @default.
- W1978083919 date "1996-01-01" @default.
- W1978083919 modified "2023-10-16" @default.
- W1978083919 title "Na<sup>+</sup> Ions Binding to the Bradykinin B<sub>2</sub> Receptor Suppress Agonist-Independent Receptor Activation" @default.
- W1978083919 cites W1445935732 @default.
- W1978083919 cites W1489775551 @default.
- W1978083919 cites W1512898428 @default.
- W1978083919 cites W1522925775 @default.
- W1978083919 cites W1539774089 @default.
- W1978083919 cites W1967534234 @default.
- W1978083919 cites W1974146850 @default.
- W1978083919 cites W2012148864 @default.
- W1978083919 cites W2030719511 @default.
- W1978083919 cites W2036379629 @default.
- W1978083919 cites W2135574054 @default.
- W1978083919 doi "https://doi.org/10.1021/bi961163w" @default.
- W1978083919 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8873604" @default.
- W1978083919 hasPublicationYear "1996" @default.
- W1978083919 type Work @default.
- W1978083919 sameAs 1978083919 @default.
- W1978083919 citedByCount "39" @default.
- W1978083919 countsByYear W19780839192012 @default.
- W1978083919 countsByYear W19780839192014 @default.
- W1978083919 countsByYear W19780839192015 @default.
- W1978083919 countsByYear W19780839192016 @default.
- W1978083919 countsByYear W19780839192017 @default.
- W1978083919 countsByYear W19780839192018 @default.
- W1978083919 countsByYear W19780839192019 @default.
- W1978083919 countsByYear W19780839192023 @default.
- W1978083919 crossrefType "journal-article" @default.
- W1978083919 hasAuthorship W1978083919A5035742650 @default.
- W1978083919 hasAuthorship W1978083919A5052474412 @default.
- W1978083919 hasAuthorship W1978083919A5055302959 @default.
- W1978083919 hasAuthorship W1978083919A5075814041 @default.
- W1978083919 hasConcept C125268873 @default.
- W1978083919 hasConcept C12554922 @default.
- W1978083919 hasConcept C126322002 @default.
- W1978083919 hasConcept C134018914 @default.
- W1978083919 hasConcept C153939273 @default.
- W1978083919 hasConcept C170493617 @default.
- W1978083919 hasConcept C185592680 @default.
- W1978083919 hasConcept C2778938600 @default.
- W1978083919 hasConcept C2779591629 @default.
- W1978083919 hasConcept C55493867 @default.
- W1978083919 hasConcept C71924100 @default.
- W1978083919 hasConcept C75184817 @default.
- W1978083919 hasConcept C86803240 @default.
- W1978083919 hasConcept C89951826 @default.
- W1978083919 hasConceptScore W1978083919C125268873 @default.
- W1978083919 hasConceptScore W1978083919C12554922 @default.
- W1978083919 hasConceptScore W1978083919C126322002 @default.
- W1978083919 hasConceptScore W1978083919C134018914 @default.
- W1978083919 hasConceptScore W1978083919C153939273 @default.
- W1978083919 hasConceptScore W1978083919C170493617 @default.
- W1978083919 hasConceptScore W1978083919C185592680 @default.
- W1978083919 hasConceptScore W1978083919C2778938600 @default.
- W1978083919 hasConceptScore W1978083919C2779591629 @default.
- W1978083919 hasConceptScore W1978083919C55493867 @default.
- W1978083919 hasConceptScore W1978083919C71924100 @default.
- W1978083919 hasConceptScore W1978083919C75184817 @default.
- W1978083919 hasConceptScore W1978083919C86803240 @default.
- W1978083919 hasConceptScore W1978083919C89951826 @default.
- W1978083919 hasIssue "41" @default.
- W1978083919 hasLocation W19780839191 @default.
- W1978083919 hasLocation W19780839192 @default.
- W1978083919 hasOpenAccess W1978083919 @default.
- W1978083919 hasPrimaryLocation W19780839191 @default.
- W1978083919 hasRelatedWork W1528146981 @default.
- W1978083919 hasRelatedWork W1979557307 @default.
- W1978083919 hasRelatedWork W1995516004 @default.
- W1978083919 hasRelatedWork W2027913950 @default.
- W1978083919 hasRelatedWork W2050137519 @default.
- W1978083919 hasRelatedWork W2063619316 @default.
- W1978083919 hasRelatedWork W2068497320 @default.
- W1978083919 hasRelatedWork W2076970302 @default.
- W1978083919 hasRelatedWork W2086238867 @default.
- W1978083919 hasRelatedWork W2505875904 @default.
- W1978083919 hasVolume "35" @default.
- W1978083919 isParatext "false" @default.
- W1978083919 isRetracted "false" @default.
- W1978083919 magId "1978083919" @default.
- W1978083919 workType "article" @default.