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- W1978108571 abstract "In a stimulated emission depletion (STED) microscope the region from which a fluorophore can spontaneously emit shrinks with the continued STED beam action after the excitation event. This fact has been recently used to implement a versatile, simple and cheap STED microscope that uses a pulsed excitation beam, a STED beam running in continuous-wave (CW) and a time-gated detection: By collecting only the delayed (with respect to the excitation events) fluorescence, the STED beam intensity needed for obtaining a certain spatial resolution strongly reduces, which is fundamental to increase live cell imaging compatibility. This new STED microscopy implementation, namely gated CW-STED, is in essence limited (only) by the reduction of the signal associated with the time-gated detection. Here we show the recent advances in gated CW-STED microscopy and related methods. We show that the time-gated detection can be substituted by more efficient computational methods when the arrival-times of all fluorescence photons are provided." @default.
- W1978108571 created "2016-06-24" @default.
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- W1978108571 date "2015-03-09" @default.
- W1978108571 modified "2023-10-15" @default.
- W1978108571 title "The importance of the photon arrival times in STED microscopy" @default.
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- W1978108571 doi "https://doi.org/10.1117/12.2081553" @default.
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