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• W1978152458 abstract "A study was conducted to evaluate the potential of immature oocytes from the ovaries of yak. The effects of maturation times (23–30 h), hormones (FSH, LH, E2), sera (fetal calf serum, FCS, or oestrous cow serum, ECS) and culture system (co-culture with granulosa cell, GC, or with bovine oviduct epithelia cells, BOEC, tissue culture medium, TCM, 199 absence co-culture cell, and synthetic oviduct fluid with amino acids, SOFaa) on the in vitro development of in vitro matured and fertilised yak oocytes were examined. Immature oocytes surrounded with compacted cumulus cell were cultured for 23–30 h in TCM 199 supplemented with 10% FCS and hormones. In vitro fertilisation (IVF) was performed with frozen-thawed, caffeine and heparin treated spermatozoa from Datong yak. Oocytes were incubated with 1×106/ml spermatozoa for 16–18 h and then cultured in co-culture system with GC or BOEC and/or SOFaa for 7–8 days, respectively. Cleavage and development to blastocysts were recorded on days 2 and 8, respectively, after the start of culture. 27–28 h was superior to other culture times and addition hormones (FSH, LH, E2) were superior to absence either of them for oocyte maturation. The best maturation of oocyte in TCM 199 with 5.0 mg/L LH, 0.5 mg/L FSH, 1 mg/L E2. FCS was superior to ECS for development to blastocysts. Co-culture with GC was superior to SOFaa and TCM 199 absence co-culture cell for cleavage, development to blastocysts. The results show that choice of culture conditions has marked effect on the development of in vitro matured and fertilised yak oocytes. The present results indicate that the co-culture with GCs is the most important factor for IVF to development into blastocysts of yak oocytes matured in vitro." @default.
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• W1978152458 date "2012-12-01" @default.
• W1978152458 modified "2023-10-16" @default.
• W1978152458 title "Efficiency of in vitro embryo production of yak (Bos grunniens) cultured in different maturation and culture conditions" @default.
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• W1978152458 doi "https://doi.org/10.1080/09712119.2012.692327" @default.
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