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- W1978232488 abstract "Diffuse reflectance was applied to the biomedical studies (muscles, cardiac tissues etc.) in a form of either a directpseudo-optical spectrum or its second derivative. The first derivative adopts advantages of both direct spectrum (highsignal-to-noise ratio) and its second derivative (simplifying the consideration of light scattering contribution, S). Incontrast to spectrophotometry of solutions, diffuse reflectance application to the analysis of turbid mediumchromophores leads to non-trivial problems of contribution of light scattering, the choice of reference, and lightpathlength. Under certain conditions, the first approximation of the Taylor series of S results in the known lineardependence of S on wavelength in the 650-1050 nm wavelength range. Then the light scattering contribution to the firstderivative becomes a wavelength-independent offset. In contrast to the second derivative, the information on lightscattering inside the tissue is not lost. Effect of reference on the measured spectra becomes negligible. Application of thefirst derivative allowed (i) determination of NIR light pathlength in muscle tissue, and (ii) quantification of hemoglobin+ myoglobin absolute concentration (in mM) in cardiac tissue during open-heart surgery. The first derivative approachmay in general be applied to any chromophores in turbid (biological) media." @default.
- W1978232488 created "2016-06-24" @default.
- W1978232488 creator A5072910948 @default.
- W1978232488 date "2009-02-12" @default.
- W1978232488 modified "2023-09-23" @default.
- W1978232488 title "First derivative of NIR light diffuse reflectance spectra as an approach to analyze muscle tissue chromophores and light pathlength" @default.
- W1978232488 doi "https://doi.org/10.1117/12.807716" @default.
- W1978232488 hasPublicationYear "2009" @default.
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