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- W1978233556 abstract "Papaya ( Carica papaya L.) cell wall matrix polysaccharides are modified as the fruit starts to soften during ripening and an endoxylanase is expressed that may play a role in the softening process. Endoxylanase gene expression, protein amount and activity were determined in papaya cultivars that differ in softening pattern and in one cultivar where softening was modified by the ethylene receptor inhibitor 1‐methylcyclopropene (1‐MCP). Antibodies to the endoxylanase catalytic domain were used to determine protein accumulation. The three papaya varieties used in the study, ‘Line 8’, ‘Sunset’, and ‘Line 4‐16’, differed in softening pattern, respiration rate, ethylene production and showed similar parallel relationships during ripening and softening in endoxylanase expression, protein level and activity. When fruit of the three papaya varieties showed the respiratory climacteric and started to soften, the level of endoxylanase gene expression increased and this increase was related to the amount of endoxylanase protein at 32 kDa and its activity. Fruit when treated at less than 10% skin yellow stage with 1‐MCP showed a significant delay in the respiratory climacteric and softening, and reduced ethylene production, and when ripe was firmer and had a ‘rubbery’ texture. The 1‐MCP‐treated fruit that had the ‘rubbery’ texture showed suppressed endoxylanase gene expression, protein and enzymatic activity. Little or no delay occurred between endoxylanase gene expression and the appearance of activity during posttranslational processing from 65 to 32 kDa. The close relationship between endoxylanase gene expression, protein accumulation and activity in different varieties and the failure of the 1‐MCP‐treated fruit to fully soften, supported de novo synthesis of endoxylanase, rapid posttranslation processing and a role in papaya fruit softening." @default.
- W1978233556 created "2016-06-24" @default.
- W1978233556 creator A5024104013 @default.
- W1978233556 creator A5080868122 @default.
- W1978233556 date "2007-08-15" @default.
- W1978233556 modified "2023-10-10" @default.
- W1978233556 title "Papaya fruit softening, endoxylanase gene expression, protein and activity" @default.
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- W1978233556 doi "https://doi.org/10.1111/j.1399-3054.2007.00967.x" @default.
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