FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978251954> ?p ?o ?g. }

• W1978251954 endingPage "8575" @default.
• W1978251954 startingPage "8571" @default.
• W1978251954 abstract "Topoisomerase IV (Topo IV), encoded by parC and parE, is required for partition of the daughter chromosomes in Escherichia coli. This enzyme is likely responsible for decatenating the linked daughter chromosomes after replication. In this report, we have examined the action of Topo IV in both pBR322 and oriC DNA replication reconstituted in vitro with purified proteins. Gyrase fails to decatenate the linked daughter molecules under any condition in the oriC system and at physiological salt concentrations in the pBR322 system, whereas Topo IV stimulates generation of monomer product DNA by 7- to 10-fold. Topo IV-catalyzed decatenation of isolated multiply linked DNA dimers was relatively insensitive to salt; it proceeded at 14% of the maximal rate even in the presence of 800 mM potassium glutamate. In contrast, decatenation in vitro by gyrase was inhibited completely under these conditions. Pulse-chase analysis indicated that Topo IV-catalyzed resolution of linked daughter DNA molecules occurred prior to completion of DNA replication, such that multiply linked daughter molecules did not arise. These results suggest that during DNA replication, gyrase acts primarily to relieve accumulated positive supercoiling and Topo IV acts to segregate the daughter chromosomes." @default.
• W1978251954 created "2016-06-24" @default.
• W1978251954 creator A5032393123 @default.
• W1978251954 creator A5068066930 @default.
• W1978251954 date "1993-09-15" @default.
• W1978251954 modified "2023-09-25" @default.
• W1978251954 title "Decatenation activity of topoisomerase IV during oriC and pBR322 DNA replication in vitro." @default.
• W1978251954 cites W1485021232 @default.
• W1978251954 cites W1504479686 @default.
• W1978251954 cites W1533084129 @default.
• W1978251954 cites W1557336515 @default.
• W1978251954 cites W1560489569 @default.
• W1978251954 cites W1560669282 @default.
• W1978251954 cites W1566869874 @default.
• W1978251954 cites W1575294415 @default.
• W1978251954 cites W1585921788 @default.
• W1978251954 cites W1797816540 @default.
• W1978251954 cites W1969049566 @default.
• W1978251954 cites W1992889417 @default.
• W1978251954 cites W2002237446 @default.
• W1978251954 cites W2002486322 @default.
• W1978251954 cites W2006156736 @default.
• W1978251954 cites W2059678906 @default.
• W1978251954 cites W2060417297 @default.
• W1978251954 cites W2066633500 @default.
• W1978251954 cites W2075251869 @default.
• W1978251954 cites W2400924469 @default.
• W1978251954 cites W80165809 @default.
• W1978251954 doi "https://doi.org/10.1073/pnas.90.18.8571" @default.
• W1978251954 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/47399" @default.
• W1978251954 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8104339" @default.
• W1978251954 hasPublicationYear "1993" @default.
• W1978251954 type Work @default.
• W1978251954 sameAs 1978251954 @default.
• W1978251954 citedByCount "134" @default.
• W1978251954 countsByYear W19782519542012 @default.
• W1978251954 countsByYear W19782519542013 @default.
• W1978251954 countsByYear W19782519542014 @default.
• W1978251954 countsByYear W19782519542015 @default.
• W1978251954 countsByYear W19782519542016 @default.
• W1978251954 countsByYear W19782519542017 @default.
• W1978251954 countsByYear W19782519542018 @default.
• W1978251954 countsByYear W19782519542019 @default.
• W1978251954 countsByYear W19782519542020 @default.
• W1978251954 countsByYear W19782519542021 @default.
• W1978251954 countsByYear W19782519542022 @default.
• W1978251954 countsByYear W19782519542023 @default.
• W1978251954 crossrefType "journal-article" @default.
• W1978251954 hasAuthorship W1978251954A5032393123 @default.
• W1978251954 hasAuthorship W1978251954A5068066930 @default.
• W1978251954 hasBestOaLocation W19782519542 @default.
• W1978251954 hasConcept C104317684 @default.
• W1978251954 hasConcept C147897179 @default.
• W1978251954 hasConcept C153911025 @default.
• W1978251954 hasConcept C167125095 @default.
• W1978251954 hasConcept C188036394 @default.
• W1978251954 hasConcept C2777112418 @default.
• W1978251954 hasConcept C547475151 @default.
• W1978251954 hasConcept C552990157 @default.
• W1978251954 hasConcept C55493867 @default.
• W1978251954 hasConcept C58167604 @default.
• W1978251954 hasConcept C64241487 @default.
• W1978251954 hasConcept C73573662 @default.
• W1978251954 hasConcept C86803240 @default.
• W1978251954 hasConcept C98086919 @default.
• W1978251954 hasConceptScore W1978251954C104317684 @default.
• W1978251954 hasConceptScore W1978251954C147897179 @default.
• W1978251954 hasConceptScore W1978251954C153911025 @default.
• W1978251954 hasConceptScore W1978251954C167125095 @default.
• W1978251954 hasConceptScore W1978251954C188036394 @default.
• W1978251954 hasConceptScore W1978251954C2777112418 @default.
• W1978251954 hasConceptScore W1978251954C547475151 @default.
• W1978251954 hasConceptScore W1978251954C552990157 @default.
• W1978251954 hasConceptScore W1978251954C55493867 @default.
• W1978251954 hasConceptScore W1978251954C58167604 @default.
• W1978251954 hasConceptScore W1978251954C64241487 @default.
• W1978251954 hasConceptScore W1978251954C73573662 @default.
• W1978251954 hasConceptScore W1978251954C86803240 @default.
• W1978251954 hasConceptScore W1978251954C98086919 @default.
• W1978251954 hasIssue "18" @default.
• W1978251954 hasLocation W19782519541 @default.
• W1978251954 hasLocation W19782519542 @default.
• W1978251954 hasLocation W19782519543 @default.
• W1978251954 hasLocation W19782519544 @default.
• W1978251954 hasOpenAccess W1978251954 @default.
• W1978251954 hasPrimaryLocation W19782519541 @default.
• W1978251954 hasRelatedWork W1533084129 @default.
• W1978251954 hasRelatedWork W1978251954 @default.
• W1978251954 hasRelatedWork W1979474715 @default.
• W1978251954 hasRelatedWork W1992889417 @default.
• W1978251954 hasRelatedWork W2001243940 @default.
• W1978251954 hasRelatedWork W2001764918 @default.
• W1978251954 hasRelatedWork W2149467013 @default.
• W1978251954 hasRelatedWork W2320865961 @default.
• W1978251954 hasRelatedWork W2891754900 @default.
• W1978251954 hasRelatedWork W3129465602 @default.
• W1978251954 hasVolume "90" @default.
• W1978251954 isParatext "false" @default.

• next