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- W1978272011 abstract "Agrobacterium tumefaciens strain AB2/73 isolated from Lippia canescens has been described as a limited-host-range strain. Its tumor-inducing (Ti) plasmid has been found to lack DNA homology to known T-DNAs (L. Unger, S. F. Ziegler, G. A. Huffman, V. C. Knauf, R. Peet, L. W. Moore, M. P. Gordon, and E. W. Nester. J. Bacteriol. 164:723–730, 1985). We have isolated a T-DNA from AB2/73 by using a heterologous border sequence as a probe. The AB2/73 T-DNA sequence (3,504 bp) is flanked by canonical border sequences, has no detectable DNA homology with other T-DNAs, and contains only two genes: lsn ( Lippia strain nopaline synthaselike gene) and lso ( Lippia strain oncogene). The lso gene induces nondif-ferentiating tumors on a limited number of hosts when transferred by a Ti plasmid from a wide-host-range strain. Part of the predicted Lso protein is weakly homologous to other Agrobacterium oncoproteins encoded by rolB, rolB TR , orf13, gene e, gene 5, and gene 3′. A 28-kb fragment corresponding to the virA to virE region was cloned by using a heterologous vir fragment as probe. The AB2/73 vir region is homologous to most of the C58 virulence region; however, the virA gene is most related to the virA gene of the Agrobacterium vitis limited-host-range strain Ag162." @default.
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- W1978272011 date "1998-05-01" @default.
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- W1978272011 title "A T-DNA from the <i>Agrobacterium tumefaciens</i> Limited-Host-Range Strain AB2/73 Contains a Single Oncogene" @default.
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- W1978272011 doi "https://doi.org/10.1094/mpmi.1998.11.5.335" @default.
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