Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978281245> ?p ?o ?g. }
- W1978281245 endingPage "437" @default.
- W1978281245 startingPage "432" @default.
- W1978281245 abstract "Currently, the species Bifidobacterium animalis consists of two subspecies, B. animalis subsp. lactis and B. animalis subsp. animalis. Among these two subspecies, B. animalis subsp. lactis is especially important because it is widely used in the manufacture of probiotic dairy products. The application of these microbes in the food industry demands fast, accurate and low cost methods to differentiate between species and strains. Although various genotypic methods have been employed to discriminate between these two subspecies, they are not easily adapted for rapid identification in the industry. The purpose of this study was to evaluate the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to differentiate between the two subspecies of B. animalis, and for discrimination at strain level. We identified twenty-three strains of B. animalis at subspecies and strain level by genotypic methods and by proteomics using MALDI-TOF MS. The proteomics identification by MALDI-TOF was nearly identical to that obtained by genotypic identification using comparison of tuf and atpD gene sequences, and single-nucleotide polymorphisms (SNPs), insertions, and deletions (INDELs). We identified four protein markers, L1, L2, A1, and A2, which are useful for discriminating between both subspecies. Proteomics identification using MALDI-TOF MS was therefore an accurate method for discriminating and identifying these bacteria. Given the speed in which this method is achieved (∼20 min including sample preparation), MALDI-TOF MS is promising as a tool for rapid discrimination of starter cultures and probiotics." @default.
- W1978281245 created "2016-06-24" @default.
- W1978281245 creator A5005921630 @default.
- W1978281245 creator A5042901531 @default.
- W1978281245 creator A5083824130 @default.
- W1978281245 creator A5091664668 @default.
- W1978281245 date "2012-06-01" @default.
- W1978281245 modified "2023-10-18" @default.
- W1978281245 title "Rapid discrimination of Bifidobacterium animalis subspecies by matrix-assisted laser desorption ionization-time of flight mass spectrometry" @default.
- W1978281245 cites W1979607601 @default.
- W1978281245 cites W1986006642 @default.
- W1978281245 cites W1994714458 @default.
- W1978281245 cites W1996289599 @default.
- W1978281245 cites W1996465910 @default.
- W1978281245 cites W2018774199 @default.
- W1978281245 cites W2019471474 @default.
- W1978281245 cites W2020270531 @default.
- W1978281245 cites W2032014394 @default.
- W1978281245 cites W2035135550 @default.
- W1978281245 cites W2039524673 @default.
- W1978281245 cites W2045582750 @default.
- W1978281245 cites W2045664599 @default.
- W1978281245 cites W2045745337 @default.
- W1978281245 cites W2051609547 @default.
- W1978281245 cites W2055855217 @default.
- W1978281245 cites W2057847565 @default.
- W1978281245 cites W2060149567 @default.
- W1978281245 cites W2060980739 @default.
- W1978281245 cites W2063109645 @default.
- W1978281245 cites W2063442828 @default.
- W1978281245 cites W2068719593 @default.
- W1978281245 cites W2068904931 @default.
- W1978281245 cites W2069509577 @default.
- W1978281245 cites W2086758072 @default.
- W1978281245 cites W2100117600 @default.
- W1978281245 cites W2100181567 @default.
- W1978281245 cites W2102068449 @default.
- W1978281245 cites W2106882534 @default.
- W1978281245 cites W2114352976 @default.
- W1978281245 cites W2120630118 @default.
- W1978281245 cites W2131970571 @default.
- W1978281245 cites W2139304530 @default.
- W1978281245 cites W2140884463 @default.
- W1978281245 cites W2141074855 @default.
- W1978281245 cites W2142216082 @default.
- W1978281245 cites W2148137892 @default.
- W1978281245 cites W2149476709 @default.
- W1978281245 cites W2152555172 @default.
- W1978281245 cites W2166895353 @default.
- W1978281245 doi "https://doi.org/10.1016/j.fm.2011.12.012" @default.
- W1978281245 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3297970" @default.
- W1978281245 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22365357" @default.
- W1978281245 hasPublicationYear "2012" @default.
- W1978281245 type Work @default.
- W1978281245 sameAs 1978281245 @default.
- W1978281245 citedByCount "26" @default.
- W1978281245 countsByYear W19782812452012 @default.
- W1978281245 countsByYear W19782812452013 @default.
- W1978281245 countsByYear W19782812452014 @default.
- W1978281245 countsByYear W19782812452016 @default.
- W1978281245 countsByYear W19782812452017 @default.
- W1978281245 countsByYear W19782812452018 @default.
- W1978281245 countsByYear W19782812452019 @default.
- W1978281245 countsByYear W19782812452020 @default.
- W1978281245 countsByYear W19782812452021 @default.
- W1978281245 countsByYear W19782812452022 @default.
- W1978281245 countsByYear W19782812452023 @default.
- W1978281245 crossrefType "journal-article" @default.
- W1978281245 hasAuthorship W1978281245A5005921630 @default.
- W1978281245 hasAuthorship W1978281245A5042901531 @default.
- W1978281245 hasAuthorship W1978281245A5083824130 @default.
- W1978281245 hasAuthorship W1978281245A5091664668 @default.
- W1978281245 hasBestOaLocation W19782812452 @default.
- W1978281245 hasConcept C104317684 @default.
- W1978281245 hasConcept C135763542 @default.
- W1978281245 hasConcept C150394285 @default.
- W1978281245 hasConcept C151730666 @default.
- W1978281245 hasConcept C162356407 @default.
- W1978281245 hasConcept C162711632 @default.
- W1978281245 hasConcept C178790620 @default.
- W1978281245 hasConcept C17966761 @default.
- W1978281245 hasConcept C185592680 @default.
- W1978281245 hasConcept C2777945686 @default.
- W1978281245 hasConcept C2780206646 @default.
- W1978281245 hasConcept C2780305828 @default.
- W1978281245 hasConcept C31467283 @default.
- W1978281245 hasConcept C43617362 @default.
- W1978281245 hasConcept C46111723 @default.
- W1978281245 hasConcept C523546767 @default.
- W1978281245 hasConcept C54355233 @default.
- W1978281245 hasConcept C70721500 @default.
- W1978281245 hasConcept C75280812 @default.
- W1978281245 hasConcept C86803240 @default.
- W1978281245 hasConcept C89423630 @default.
- W1978281245 hasConceptScore W1978281245C104317684 @default.
- W1978281245 hasConceptScore W1978281245C135763542 @default.
- W1978281245 hasConceptScore W1978281245C150394285 @default.
- W1978281245 hasConceptScore W1978281245C151730666 @default.