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- W1978353478 endingPage "4562" @default.
- W1978353478 startingPage "4546" @default.
- W1978353478 abstract "Abstract Between 6 and 30% of human and mouse transcripts are initiated from transposable elements. However, the promoters driving such transcriptional activity are mostly unknown. We experimentally characterized an antisense (AS) promoter in mouse L1 retrotransposons for the first time, oriented antiparallel to the coding strand of L1 open reading frame-1. We found that AS transcription is mediated by RNA polymerase II. Rapid amplification of cDNA ends cloning mapped transcription start sites adjacent to the AS promoter. We identified >100 novel fusion transcripts, of which many were conserved across divergent mouse lineages, suggesting conservation of potential functions. To evaluate whether AS L1 transcription could regulate L1 retrotransposition, we replaced portions of native open reading frame-1 in donor elements by synonymously recoded sequences. The resulting L1 elements lacked AS promoter activity and retrotransposed more frequently than endogenous L1s. Overexpression of AS L1 transcripts also reduced L1 retrotransposition. This suppression of retrotransposition was largely independent of Dicer. Our experiments shed new light on how AS fusion transcripts are initiated from endogenous L1 elements across the mouse genome. Such AS transcription can contribute substantially both to natural transcriptional variation and to endogenous regulation of L1 retrotransposition." @default.
- W1978353478 created "2016-06-24" @default.
- W1978353478 creator A5009636880 @default.
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- W1978353478 creator A5072618676 @default.
- W1978353478 creator A5081362834 @default.
- W1978353478 creator A5084832087 @default.
- W1978353478 date "2014-01-31" @default.
- W1978353478 modified "2023-10-16" @default.
- W1978353478 title "An antisense promoter in mouse L1 retrotransposon open reading frame-1 initiates expression of diverse fusion transcripts and limits retrotransposition" @default.
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- W1978353478 doi "https://doi.org/10.1093/nar/gku091" @default.