Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978355831> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W1978355831 endingPage "403" @default.
- W1978355831 startingPage "394" @default.
- W1978355831 abstract "Canine generalized progressive retinal atrophies (gPRA) are a group of degenerative retinal diseases that are a major cause of hereditary blindness in a number of dog breeds. The expressed sequence tag (EST) approach was used to identify and characterize potential candidate genes from canine retinal cDNA libraries. Both conventional and subtractive canine retinal cDNA libraries were constructed and analyzed. Differential hybridization was performed to identify abundantly retinal expressed cDNA clones. Sequences of both random and abundantly expressed clones were analyzed using GCG software and searched against GenEMBL databases. For genes of interest isolated from the libraries, Northern blotting and RT-PCR were performed to determine mRNA expression of the genes. DNA sequences from 85 differentially expressed clones and 100 random cDNAs were obtained and analyzed. A higher percentage of abundantly retina-expressed clones showed homology to database sequences compared with random clones (72 versus 43%). Five retinal genes and 2 anonymous retinal ESTs were selected to analyze mRNA expression. The five known genes, namely HRG4/unc119, cGMP-PDEA, transducin 1A, opsin, and sFRP2 showed retina-specific expression. In anonymous ESTs, clone p81 revealed retina-specific expression, while p3 showed expression in each of 14 canine tissues. Transcripts of the canine secreted frizzled related protein 2 (sFRP2) gene showed surprisingly high abundance in the canine retina. The isolated retinal ESTs here will be useful resources for further investigation of canine retinal function and canine genome mapping." @default.
- W1978355831 created "2016-06-24" @default.
- W1978355831 creator A5011524795 @default.
- W1978355831 creator A5053636127 @default.
- W1978355831 date "2001-03-01" @default.
- W1978355831 modified "2023-09-27" @default.
- W1978355831 title "Generation and Analysis of Canine Retinal ESTs: Isolation and Expression of Retina-Specific Gene Transcripts" @default.
- W1978355831 cites W1501393883 @default.
- W1978355831 cites W1974291147 @default.
- W1978355831 cites W1977118713 @default.
- W1978355831 cites W1977221760 @default.
- W1978355831 cites W1977605251 @default.
- W1978355831 cites W1982709013 @default.
- W1978355831 cites W1992299477 @default.
- W1978355831 cites W2001372468 @default.
- W1978355831 cites W2010598119 @default.
- W1978355831 cites W2014627257 @default.
- W1978355831 cites W2015292449 @default.
- W1978355831 cites W2020902625 @default.
- W1978355831 cites W2021868178 @default.
- W1978355831 cites W2033332981 @default.
- W1978355831 cites W2044562743 @default.
- W1978355831 cites W2055043387 @default.
- W1978355831 cites W2060386759 @default.
- W1978355831 cites W2064304960 @default.
- W1978355831 cites W2088333455 @default.
- W1978355831 cites W2092078711 @default.
- W1978355831 cites W2153003103 @default.
- W1978355831 cites W4294216491 @default.
- W1978355831 doi "https://doi.org/10.1006/bbrc.2001.4587" @default.
- W1978355831 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11401471" @default.
- W1978355831 hasPublicationYear "2001" @default.
- W1978355831 type Work @default.
- W1978355831 sameAs 1978355831 @default.
- W1978355831 citedByCount "13" @default.
- W1978355831 crossrefType "journal-article" @default.
- W1978355831 hasAuthorship W1978355831A5011524795 @default.
- W1978355831 hasAuthorship W1978355831A5053636127 @default.
- W1978355831 hasConcept C104317684 @default.
- W1978355831 hasConcept C149034497 @default.
- W1978355831 hasConcept C150194340 @default.
- W1978355831 hasConcept C153911025 @default.
- W1978355831 hasConcept C165525559 @default.
- W1978355831 hasConcept C169760540 @default.
- W1978355831 hasConcept C187882448 @default.
- W1978355831 hasConcept C189482833 @default.
- W1978355831 hasConcept C196436469 @default.
- W1978355831 hasConcept C2777093970 @default.
- W1978355831 hasConcept C2780827179 @default.
- W1978355831 hasConcept C54355233 @default.
- W1978355831 hasConcept C55493867 @default.
- W1978355831 hasConcept C86803240 @default.
- W1978355831 hasConceptScore W1978355831C104317684 @default.
- W1978355831 hasConceptScore W1978355831C149034497 @default.
- W1978355831 hasConceptScore W1978355831C150194340 @default.
- W1978355831 hasConceptScore W1978355831C153911025 @default.
- W1978355831 hasConceptScore W1978355831C165525559 @default.
- W1978355831 hasConceptScore W1978355831C169760540 @default.
- W1978355831 hasConceptScore W1978355831C187882448 @default.
- W1978355831 hasConceptScore W1978355831C189482833 @default.
- W1978355831 hasConceptScore W1978355831C196436469 @default.
- W1978355831 hasConceptScore W1978355831C2777093970 @default.
- W1978355831 hasConceptScore W1978355831C2780827179 @default.
- W1978355831 hasConceptScore W1978355831C54355233 @default.
- W1978355831 hasConceptScore W1978355831C55493867 @default.
- W1978355831 hasConceptScore W1978355831C86803240 @default.
- W1978355831 hasIssue "2" @default.
- W1978355831 hasLocation W19783558311 @default.
- W1978355831 hasLocation W19783558312 @default.
- W1978355831 hasOpenAccess W1978355831 @default.
- W1978355831 hasPrimaryLocation W19783558311 @default.
- W1978355831 hasRelatedWork W1464584225 @default.
- W1978355831 hasRelatedWork W1531528770 @default.
- W1978355831 hasRelatedWork W2347422362 @default.
- W1978355831 hasRelatedWork W2351351981 @default.
- W1978355831 hasRelatedWork W2359282315 @default.
- W1978355831 hasRelatedWork W2380306570 @default.
- W1978355831 hasRelatedWork W262379139 @default.
- W1978355831 hasRelatedWork W3140273298 @default.
- W1978355831 hasRelatedWork W3148398443 @default.
- W1978355831 hasRelatedWork W886888406 @default.
- W1978355831 hasVolume "282" @default.
- W1978355831 isParatext "false" @default.
- W1978355831 isRetracted "false" @default.
- W1978355831 magId "1978355831" @default.
- W1978355831 workType "article" @default.