Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978372216> ?p ?o ?g. }
- W1978372216 endingPage "767" @default.
- W1978372216 startingPage "753" @default.
- W1978372216 abstract "Imaging of living cells and tissue is now common in many fields of the life and physical sciences, and is instrumental in revealing a great deal about cellular dynamics and function. It is crucial when performing such experiments that cell viability is at the forefront of any measurement to ensure that the physiological and biological processes that are under investigation are not altered in any way. Many cells and tissues are not normally exposed to light during their life cycle, so it is important for microscopy applications to minimize light exposure, which can cause phototoxicity. To ensure minimal light exposure, it is crucial that microscope systems are optimized to collect as much light as possible. This can be achieved using superior-quality optical components and state-of-the-art detectors. This Commentary discusses how to set up a suitable environment on the microscope stage to maintain living cells. There is also a focus on general and imaging-platform-specific ways to optimize the efficiency of light throughput and detection. With an efficient optical microscope and a good detector, the light exposure can be minimized during live-cell imaging, thus minimizing phototoxicity and maintaining cell viability. Brief suggestions for useful microscope accessories as well as available fluorescence tools are also presented. Finally, a flow chart is provided to assist readers in choosing the appropriate imaging platform for their experimental systems." @default.
- W1978372216 created "2016-06-24" @default.
- W1978372216 creator A5021503872 @default.
- W1978372216 creator A5036995830 @default.
- W1978372216 creator A5060670410 @default.
- W1978372216 creator A5068414716 @default.
- W1978372216 date "2009-03-15" @default.
- W1978372216 modified "2023-10-16" @default.
- W1978372216 title "Live-cell microscopy – tips and tools" @default.
- W1978372216 cites W1519175078 @default.
- W1978372216 cites W1530269466 @default.
- W1978372216 cites W1534817517 @default.
- W1978372216 cites W1539090868 @default.
- W1978372216 cites W1541050587 @default.
- W1978372216 cites W1557714923 @default.
- W1978372216 cites W1564351816 @default.
- W1978372216 cites W1601798363 @default.
- W1978372216 cites W1604072353 @default.
- W1978372216 cites W1890786566 @default.
- W1978372216 cites W1958433286 @default.
- W1978372216 cites W1968666129 @default.
- W1978372216 cites W1976136773 @default.
- W1978372216 cites W1979038578 @default.
- W1978372216 cites W1984387240 @default.
- W1978372216 cites W1985701554 @default.
- W1978372216 cites W1988326309 @default.
- W1978372216 cites W1989239695 @default.
- W1978372216 cites W1993759736 @default.
- W1978372216 cites W1996657515 @default.
- W1978372216 cites W1998337135 @default.
- W1978372216 cites W2000259686 @default.
- W1978372216 cites W2001752971 @default.
- W1978372216 cites W2010181298 @default.
- W1978372216 cites W2013990977 @default.
- W1978372216 cites W2016240992 @default.
- W1978372216 cites W2016548049 @default.
- W1978372216 cites W2020472510 @default.
- W1978372216 cites W2021027041 @default.
- W1978372216 cites W2022912430 @default.
- W1978372216 cites W2026427394 @default.
- W1978372216 cites W2027429271 @default.
- W1978372216 cites W2027542725 @default.
- W1978372216 cites W2030667313 @default.
- W1978372216 cites W2038632343 @default.
- W1978372216 cites W2039210066 @default.
- W1978372216 cites W2045006795 @default.
- W1978372216 cites W2048209624 @default.
- W1978372216 cites W2055864348 @default.
- W1978372216 cites W2064023205 @default.
- W1978372216 cites W2067640792 @default.
- W1978372216 cites W2069664827 @default.
- W1978372216 cites W2073396033 @default.
- W1978372216 cites W2079075712 @default.
- W1978372216 cites W2080836071 @default.
- W1978372216 cites W2083288707 @default.
- W1978372216 cites W2093030333 @default.
- W1978372216 cites W2095103362 @default.
- W1978372216 cites W2097821935 @default.
- W1978372216 cites W2099426030 @default.
- W1978372216 cites W2099546976 @default.
- W1978372216 cites W2104954806 @default.
- W1978372216 cites W2115992503 @default.
- W1978372216 cites W2118726157 @default.
- W1978372216 cites W2120959357 @default.
- W1978372216 cites W2122281782 @default.
- W1978372216 cites W2123555845 @default.
- W1978372216 cites W2124733101 @default.
- W1978372216 cites W2126376309 @default.
- W1978372216 cites W2126629698 @default.
- W1978372216 cites W2131421679 @default.
- W1978372216 cites W2134120396 @default.
- W1978372216 cites W2135374895 @default.
- W1978372216 cites W2142508738 @default.
- W1978372216 cites W2143094150 @default.
- W1978372216 cites W2143611296 @default.
- W1978372216 cites W2147607085 @default.
- W1978372216 cites W2148493274 @default.
- W1978372216 cites W2149198894 @default.
- W1978372216 cites W2153319785 @default.
- W1978372216 cites W2154966567 @default.
- W1978372216 cites W2157207470 @default.
- W1978372216 cites W2159828012 @default.
- W1978372216 cites W2162332322 @default.
- W1978372216 cites W2165381688 @default.
- W1978372216 cites W2165853302 @default.
- W1978372216 cites W2413150078 @default.
- W1978372216 cites W3141868988 @default.
- W1978372216 cites W362058171 @default.
- W1978372216 cites W4210274784 @default.
- W1978372216 cites W4244013499 @default.
- W1978372216 cites W4249336316 @default.
- W1978372216 cites W4252713891 @default.
- W1978372216 cites W4253526749 @default.
- W1978372216 cites W99116975 @default.
- W1978372216 doi "https://doi.org/10.1242/jcs.033837" @default.
- W1978372216 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19261845" @default.
- W1978372216 hasPublicationYear "2009" @default.
- W1978372216 type Work @default.