FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W197837260> ?p ?o ?g. }

• W197837260 abstract "The global demand for energy is projected to increase by more than thirty percent by 2035, but the supply of fossil fuels is finite and alternative energy sources must be explored. The search for the ultimate bioenergy source, one that is both sustainable and cost-effective, has attracted the attention of the fuel industry and governments worldwide. In particular, interest in microalgal-derived biofuels has increased rapidly and microalgal production systems have been suggested as a viable solution to the issue of fuel supply, as well as contributing to a reduction in carbon emissions to minimize climate change effects (Stephens et al. 2010a; Wijffels and Barbosa, 2010). The gap between microalgae technology and oil-based energy is mainly represented by production costs such as algae cultivation, harvesting and extraction. An improvement in microalgae-to-biomass conversion efficiency is therefore essential to make the system profitable (Dekker and Boekema, 2005; Jansson, 2000). Many efforts to improve microalgae biomass yield have focused on photosynthetic efficiency, though progress has been slow. This is mainly due to the many parameters that need to be optimised such as exposure to light, availability of nutrients, bioreactor design, strain selection and so on. Current screening capacities are limited due to large volume culture methods and the lack of accurate techniques for the high-throughput phenotypic analyses. It is therefore critical that better technologies for screening and optimisation of high-value strains are developed in order to meet the urgent need for alternative fuel supplies. To this end, this project has examined the feasibility of strain development ofChlamydomonas reinhardtii grown in microculture format and explored the potential of high-throughput methods to analyse important parameters such as antenna size, energy loss and biomass yield. Chapter one provides an overview of the photosynthesis generally as well as a more detailed introduction concerning components of the light reaction and how these may be altered to increase biomass yield. The specific aims and significance of the thesis are also presented. Chapter two investigates the physical limitations on maximal growth rate in microculture, including light penetration and intensity, path length, shaking speed, cell density and volume. From this, it became clear that the existing technique for the estimation of biomass yield was not suitable for small volumes. Hence, chapter three presents a novel method based on flow cytometry to accurately estimate biomass in small volume cultures (Chioccioli et al., in review). This method is  amenable to high-throughput analyses and is therefore suitable for the large-scale screening and optimisation of microalgae strains. Chapter four explores the use of flow cytometry to analyse a range of other important parameters. Small antennae mutants were identified based on low fluorescence as a means to quickly screen large numbers of putative mutants following mutagenesis. Dynamic regulation of chlorophyll content and growth cycle in response to different light intensities was also analysed via flow cytometry in order to identify the optimal light intensity for biomass accumulation. Finally, we applied these data to investigate the response of wild-type and small antennae mutant Stm3LR3 to varying light intensities over time and again used flow cytometry as well as quantitative real time PCR in order to analyse the regulation of the light capture and biomass yield. Chapter five provides a summary of the key conclusions of the thesis. Overall, this thesis has provided a proof-of-concept for the scale-up of microalgae phenotypic analysis based on flow cytometry as the principal assay. This technique has provided an accurate high-throughput method to monitor a range of biological processes including biomass yield, antennae regulation, growth cycle dynamics and light regulation. The application of these findings lies in large-scale microalgae strain development and will therefore serve to advance the identification of novel, high quantity biomass-producing strains. The optimisation of biomass production is important not just for the development of biofuels such as oil, methane, ethanol and hydrogen, but also for the development of other high-value biomass products, biomass animal and fish feeds, and for targeted protein expression." @default.
• W197837260 created "2016-06-24" @default.
• W197837260 creator A5009616550 @default.
• W197837260 date "2013-02-01" @default.
• W197837260 modified "2023-09-27" @default.
• W197837260 title "Exploring new strategies for a more accurate estimation of microalgae growth rate and light harvesting complexes antennae size" @default.
• W197837260 hasPublicationYear "2013" @default.
• W197837260 type Work @default.
• W197837260 sameAs 197837260 @default.
• W197837260 citedByCount "0" @default.
• W197837260 crossrefType "journal-article" @default.
• W197837260 hasAuthorship W197837260A5009616550 @default.
• W197837260 hasConcept C115540264 @default.
• W197837260 hasConcept C127413603 @default.
• W197837260 hasConcept C150903083 @default.
• W197837260 hasConcept C156380964 @default.
• W197837260 hasConcept C159403789 @default.
• W197837260 hasConcept C161790260 @default.
• W197837260 hasConcept C162324750 @default.
• W197837260 hasConcept C175605778 @default.
• W197837260 hasConcept C183696295 @default.
• W197837260 hasConcept C188573790 @default.
• W197837260 hasConcept C18903297 @default.
• W197837260 hasConcept C21880701 @default.
• W197837260 hasConcept C2982719155 @default.
• W197837260 hasConcept C39432304 @default.
• W197837260 hasConcept C52896960 @default.
• W197837260 hasConcept C53991642 @default.
• W197837260 hasConcept C548081761 @default.
• W197837260 hasConcept C55493867 @default.
• W197837260 hasConcept C68189081 @default.
• W197837260 hasConcept C86803240 @default.
• W197837260 hasConceptScore W197837260C115540264 @default.
• W197837260 hasConceptScore W197837260C127413603 @default.
• W197837260 hasConceptScore W197837260C150903083 @default.
• W197837260 hasConceptScore W197837260C156380964 @default.
• W197837260 hasConceptScore W197837260C159403789 @default.
• W197837260 hasConceptScore W197837260C161790260 @default.
• W197837260 hasConceptScore W197837260C162324750 @default.
• W197837260 hasConceptScore W197837260C175605778 @default.
• W197837260 hasConceptScore W197837260C183696295 @default.
• W197837260 hasConceptScore W197837260C188573790 @default.
• W197837260 hasConceptScore W197837260C18903297 @default.
• W197837260 hasConceptScore W197837260C21880701 @default.
• W197837260 hasConceptScore W197837260C2982719155 @default.
• W197837260 hasConceptScore W197837260C39432304 @default.
• W197837260 hasConceptScore W197837260C52896960 @default.
• W197837260 hasConceptScore W197837260C53991642 @default.
• W197837260 hasConceptScore W197837260C548081761 @default.
• W197837260 hasConceptScore W197837260C55493867 @default.
• W197837260 hasConceptScore W197837260C68189081 @default.
• W197837260 hasConceptScore W197837260C86803240 @default.
• W197837260 hasLocation W1978372601 @default.
• W197837260 hasOpenAccess W197837260 @default.
• W197837260 hasPrimaryLocation W1978372601 @default.
• W197837260 hasRelatedWork W1508204158 @default.
• W197837260 hasRelatedWork W1975111308 @default.
• W197837260 hasRelatedWork W2009052032 @default.
• W197837260 hasRelatedWork W2010231803 @default.
• W197837260 hasRelatedWork W2050546582 @default.
• W197837260 hasRelatedWork W2208372299 @default.
• W197837260 hasRelatedWork W2314012644 @default.
• W197837260 hasRelatedWork W2585108168 @default.
• W197837260 hasRelatedWork W2585995843 @default.
• W197837260 hasRelatedWork W2621243191 @default.
• W197837260 hasRelatedWork W2790355745 @default.
• W197837260 hasRelatedWork W2913852796 @default.
• W197837260 hasRelatedWork W2951267893 @default.
• W197837260 hasRelatedWork W2996789779 @default.
• W197837260 hasRelatedWork W3009228582 @default.
• W197837260 hasRelatedWork W3014722337 @default.
• W197837260 hasRelatedWork W3096386324 @default.
• W197837260 hasRelatedWork W3138111041 @default.
• W197837260 hasRelatedWork W3167487540 @default.
• W197837260 hasRelatedWork W1466303934 @default.
• W197837260 isParatext "false" @default.
• W197837260 isRetracted "false" @default.
• W197837260 magId "197837260" @default.
• W197837260 workType "article" @default.